De Boer W R, Kruyssen F J, Wouters J T
Eur J Biochem. 1976 Feb 2;62(1):1-6. doi: 10.1111/j.1432-1033.1976.tb10090.x.
The walls of Bacillus subtilis var. niger WM, grown in a Mg2+ -limited chemostat culture (carbon source glucose, dilution rate = 0.2 h(-1), 37 degrees C, pH 7) contained 45% (w/w) teichoic acid, a polymer composed of glycerol, phosphate[ and glucose in the molar ratio 1.00:1.00:0.88, respectively. Alkaline hydrolysis of this teichoic acid yeilded 1-O-beta-glucosylglycerol phosphate (together with small amounts of glycerol phosphate0 and 13C nuclear magnetic resonance spectra of this hydrolysis product, and its derivative after alkaline phosphate treatment, confirmed that the monomeric unit was 1-O-beta-glucosylglycerol-3-phosphate. Assignment of the resonances in the spectrum of undergraded teichoic acid revealed that the polymer was a poly [(2,3) glycerol phosphate 1, glucosidically substituted on C-1 of glycerol with beta-glucose.
在Mg2+受限的恒化器培养（碳源为葡萄糖，稀释率 = 0.2 h(-1)，37℃，pH 7）中生长的枯草芽孢杆菌黑色变种WM的细胞壁含有45%（w/w）的磷壁酸，该聚合物由甘油、磷酸和葡萄糖组成，摩尔比分别为1.00:1.00:0.88。这种磷壁酸的碱性水解产生了1-O-β-葡萄糖基甘油磷酸（以及少量的甘油磷酸），该水解产物及其碱性磷酸处理后的衍生物的13C核磁共振谱证实单体单元为1-O-β-葡萄糖基甘油-3-磷酸。未降解磷壁酸谱中共振峰的归属表明该聚合物是一种聚[(2,3)甘油磷酸1]，在甘油的C-1位上被β-葡萄糖糖苷取代。
