Hazenberg M P, Pennock-Schröder A M, van de Merwe J P
Med Microbiol Immunol. 1986;175(5):299-306. doi: 10.1007/BF02126051.
Agglutinating antibodies to Coprococcus comes and three other obligately anaerobic coccoid rods from the intestinal flora are used in the diagnosis of Crohn's disease. Further studies on the pathogenetic role as well as the development of more sensitive and specific methods for detecting antibodies require extraction of the antigen fractions. Culturing methods to obtain C. comes with optimal antigen presentation and isolation of soluble antigen fractions were therefore developed. Hot water extraction of whole cells and subsequent removal of proteins with trichloroacetic acid provided a fraction that absorbed serum agglutinins, was useful for an enzyme-linked immunosorbent assay and induced agglutinating antibodies in rats.
来自肠道菌群的对粪球菌属及其他三种专性厌氧球菌样杆菌的凝集抗体被用于克罗恩病的诊断。关于其致病作用的进一步研究以及开发更灵敏和特异的抗体检测方法需要提取抗原组分。因此，开发了用于获得具有最佳抗原呈递的粪球菌属的培养方法以及可溶性抗原组分的分离方法。全细胞的热水提取以及随后用三氯乙酸去除蛋白质，得到了一种能吸收血清凝集素的组分，该组分可用于酶联免疫吸附测定，并能在大鼠体内诱导产生凝集抗体。
