Freitas L B, Rundegren J, Arnebrant T
Department of Periodontology, Centre for Oral Health Sciences, University of Lund, Malmö, Sweden.
Oral Microbiol Immunol. 1993 Dec;8(6):355-60. doi: 10.1111/j.1399-302x.1993.tb00611.x.
This study evaluated the binding of chlorhexidine and the new surface-active anti-plaque agent delmopinol hydrochloride to Streptococcus mutans and Actinobacillus actinomycetemcomitans cells with various cell surface hydrophobicities. The influence of saliva concentration on the binding of these compounds was also investigated. The radiolabeled compounds were incubated with bacteria and the cells were recovered using a centrifugal filtering technique. Delmopinol had higher binding to the hydrophilic variant strains than to the hydrophobic parent strains; chlorhexidine had higher binding to hydrophobic than to hydrophilic A. actinomycetemcomitans strains and higher binding to hydrophilic than to hydrophobic S. mutans strains. The presence of salivary films decreased the binding of both compounds. Both delmopinol and chlorhexidine had stronger affinity to A. actinomycetemcomitans cells than to S. mutans cells. At equimolar concentrations, delmopinol had a lower binding to all strains tested than chlorhexidine. The high reversibility of the delmopinol binding might be related to a higher diffusion rate and solubility compared with that of chlorhexidine. The amphiphilicity of both molecules is an important feature in their retention to S. mutans and A. actinomycetemcomitans strains of varying hydrophobicities and could play an important role in the substantivity of delmopinol or chlorhexidine in the oral cavity.
本研究评估了洗必泰以及新型表面活性防菌斑剂盐酸地莫匹醇与具有不同细胞表面疏水性的变形链球菌和伴放线放线杆菌细胞的结合情况。还研究了唾液浓度对这些化合物结合的影响。将放射性标记的化合物与细菌一起孵育，然后使用离心过滤技术回收细胞。地莫匹醇与亲水性变异菌株的结合高于与疏水性亲本菌株的结合；洗必泰与疏水性伴放线放线杆菌菌株的结合高于与亲水性菌株的结合，与亲水性变形链球菌菌株的结合高于与疏水性菌株的结合。唾液膜的存在降低了两种化合物的结合。地莫匹醇和洗必泰对伴放线放线杆菌细胞的亲和力均强于对变形链球菌细胞的亲和力。在等摩尔浓度下，地莫匹醇与所有测试菌株的结合均低于洗必泰。地莫匹醇结合的高可逆性可能与其比洗必泰更高的扩散速率和溶解度有关。两种分子的两亲性是它们在不同疏水性的变形链球菌和伴放线放线杆菌菌株上保留的重要特征，并且可能在口腔中地莫匹醇或洗必泰的持续性中起重要作用。
