某些抗磷脂抗体针对磷脂结合的β2糖蛋白I的狼疮抗凝活性。
Lupus anticoagulant activity of some antiphospholipid antibodies against phospholipid bound beta 2 glycoprotein I.
作者信息
Keeling D M, Wilson A J, Mackie I J, Isenberg D A, Machin S J
机构信息
Department of Haematology, University College and Middlesex School of Medicine, London.
出版信息
J Clin Pathol. 1993 Jul;46(7):665-7. doi: 10.1136/jcp.46.7.665.
Abstract
AIMS
To determine whether beta 2 glycoprotein I (beta 2GPI) dependent anticardiolipin (aCL) antibodies detected in solid phase enzyme linked immunosorbent assays can also have lupus anticoagulant activity.
METHODS
Six anticardiolipin antibodies were affinity purified from patients with these antibodies and lupus anticoagulant activity in their plasma.
RESULTS
The anticardiolipin antibodies bound only to anionic phospholipids in the presence of beta 2GPI and bound to beta 2GPI in the absence of phospholipids. Four out of six had lupus anticoagulant activity in the dilute Russell viper venom time test.
CONCLUSIONS
The results show that some beta 2GPI dependent aCL are lupus anticoagulants. It is unclear why only some should have lupus anticoagulant activity while others do not.
摘要
目的
确定在固相酶联免疫吸附试验中检测到的β2糖蛋白I（β2GPI）依赖性抗心磷脂（aCL）抗体是否也具有狼疮抗凝活性。
方法
从血浆中存在这些抗体且具有狼疮抗凝活性的患者中亲和纯化出六种抗心磷脂抗体。
结果
抗心磷脂抗体仅在β2GPI存在时与阴离子磷脂结合，在无磷脂时与β2GPI结合。在稀释的罗素蝰蛇毒时间试验中，六种中有四种具有狼疮抗凝活性。
结论
结果表明，一些β2GPI依赖性aCL是狼疮抗凝剂。尚不清楚为何只有一些具有狼疮抗凝活性而其他的没有。
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