Separation of anticardiolipin antibodies from lupus anticoagulant on a phospholipid-coated polystyrene column.

We describe two novel developments. The first is the preparation of a "cemented" chromatography column comprising siliconized sand-coated by and cemented together with polystyrene thus providing a large hydrophobic surface area suitable for passive antigen absorption. The second is the coating of such a matrix with phospholipid and its use for separating anticardiolipin antibodies from lupus anticoagulant activity in the plasma of a patient with the lupus obstetric syndrome. This is the first description of the separate identities of these two antibodies.