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Separation of anticardiolipin antibodies from lupus anticoagulant on a phospholipid-coated polystyrene column.

作者信息

Exner T, Sahman N, Trudinger B

机构信息

Haematology Department, Westmead Hospital, Sydney, Australia.

出版信息

Biochem Biophys Res Commun. 1988 Sep 15;155(2):1001-7. doi: 10.1016/s0006-291x(88)80596-5.

DOI:10.1016/s0006-291x(88)80596-5
PMID:3138989
Abstract

We describe two novel developments. The first is the preparation of a "cemented" chromatography column comprising siliconized sand-coated by and cemented together with polystyrene thus providing a large hydrophobic surface area suitable for passive antigen absorption. The second is the coating of such a matrix with phospholipid and its use for separating anticardiolipin antibodies from lupus anticoagulant activity in the plasma of a patient with the lupus obstetric syndrome. This is the first description of the separate identities of these two antibodies.

摘要

相似文献

1
Separation of anticardiolipin antibodies from lupus anticoagulant on a phospholipid-coated polystyrene column.
Biochem Biophys Res Commun. 1988 Sep 15;155(2):1001-7. doi: 10.1016/s0006-291x(88)80596-5.
2
Separation of lupus anticoagulant from anticardiolipin antibodies by ion-exchange and gel filtration chromatography.通过离子交换和凝胶过滤色谱法分离狼疮抗凝物与抗心磷脂抗体。
Haemostasis. 1991;21(1):25-9. doi: 10.1159/000216198.
3
The prevalence and biologic significance of lupus anticoagulant and anticardiolipin antibodies in a general obstetric population.普通产科人群中狼疮抗凝物和抗心磷脂抗体的患病率及生物学意义。
Am J Obstet Gynecol. 1989 Aug;161(2):369-73. doi: 10.1016/0002-9378(89)90522-x.
4
Anticardiolipin antibodies and lupus anticoagulants comprise separate antibody subgroups with different phospholipid binding characteristics.抗心磷脂抗体和狼疮抗凝物构成了具有不同磷脂结合特性的独立抗体亚组。
Br J Haematol. 1989 Dec;73(4):506-13. doi: 10.1111/j.1365-2141.1989.tb00289.x.
5
Immunological specificity and mechanism of action of IgG lupus anticoagulants.
Blood. 1987 Jul;70(1):69-76.
6
Anticardiolipin antibodies: their presence as a marker for lupus anticoagulant in pregnancy.抗心磷脂抗体:其作为妊娠中狼疮抗凝物标志物的存在情况。
N Z Med J. 1987 Feb 11;100(817):61-4.
7
Screening for lupus anticoagulant and anticardiolipin antibodies in women with fetal loss.对有胎儿丢失史的女性进行狼疮抗凝物和抗心磷脂抗体筛查。
J Clin Pathol. 1991 Jan;44(1):45-7. doi: 10.1136/jcp.44.1.45.
8
Lupus anticoagulant: clinical significance in anticardiolipin positive patients with systemic lupus erythematosus.狼疮抗凝物:在抗心磷脂抗体阳性的系统性红斑狼疮患者中的临床意义
Ann Rheum Dis. 1991 Aug;50(8):548-52. doi: 10.1136/ard.50.8.548.
9
Chlorpromazine-induced anticardiolipin antibodies and lupus anticoagulant: absence of thrombosis.氯丙嗪诱导的抗心磷脂抗体和狼疮抗凝物:无血栓形成。
Am J Hematol. 1988 Apr;27(4):272-5. doi: 10.1002/ajh.2830270408.
10
Binding specificity of lupus anticoagulants and anticardiolipin antibodies.狼疮抗凝物和抗心磷脂抗体的结合特异性。
Thromb Res. 1988 Dec 15;52(6):609-19. doi: 10.1016/0049-3848(88)90133-8.

引用本文的文献

1
Anti-beta2-glycoprotein I (beta2GPI) monoclonal antibodies with lupus anticoagulant-like activity enhance the beta2GPI binding to phospholipids.具有狼疮抗凝物样活性的抗β2糖蛋白I(β2GPI)单克隆抗体可增强β2GPI与磷脂的结合。
J Clin Invest. 1997 May 1;99(9):2260-8. doi: 10.1172/JCI119401.
2
Lupus anticoagulant activity of some antiphospholipid antibodies against phospholipid bound beta 2 glycoprotein I.某些抗磷脂抗体针对磷脂结合的β2糖蛋白I的狼疮抗凝活性。
J Clin Pathol. 1993 Jul;46(7):665-7. doi: 10.1136/jcp.46.7.665.
3
Anti-beta 2-glycoprotein I antibodies and thrombosis.
抗β2-糖蛋白I抗体与血栓形成
Clin Rev Allergy Immunol. 1995 Spring;13(1):67-72. doi: 10.1007/BF02772250.
4
Antigenic specificities of "antiphospholipid" autoantibodies.“抗磷脂”自身抗体的抗原特异性
Springer Semin Immunopathol. 1994;16(2-3):211-22. doi: 10.1007/BF00197518.
5
Neurological disease associated with anticardiolipin antibodies in patients without systemic lupus erythematosus: clinical and immunological features.无系统性红斑狼疮患者中与抗心磷脂抗体相关的神经系统疾病:临床和免疫学特征
J Neurol. 1991 Oct;238(7):401-7. doi: 10.1007/BF00319860.
6
Lupus anticoagulant activity of autoimmune antiphospholipid antibodies is dependent upon beta 2-glycoprotein I.自身免疫性抗磷脂抗体的狼疮抗凝活性依赖于β2-糖蛋白I。
J Clin Invest. 1992 Sep;90(3):1100-4. doi: 10.1172/JCI115926.
7
Phospholipid binding of antiphospholipid antibodies and placental anticoagulant protein.抗磷脂抗体与胎盘抗凝蛋白的磷脂结合
J Clin Immunol. 1992 Jan;12(1):27-35. doi: 10.1007/BF00918270.