Perry R L, Carrig C B, Williams J F, Johnson C A, Kaneene J B
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
Lab Anim Sci. 1993 Dec;43(6):586-93.
This study presents the first report of the identification of anatomic structures on survey radiographs of the abdomen of the rat, along with detailed barium contrast studies of the gastrointestinal tract in normal rats. The rats (49 to 112 days old) were chemically restrained with a combination of 0.71 mg ketamine hydrochloride/g of body weight and 0.5 mg of acetylpromazine maleate injected intramuscularly. Following sedation, a 36% (wt/vol) micropulverized barium sulfate suspension was administered via a stomach tube at a dose of 0.02 ml/g of body weight. The radiographic features of the gastrointestinal tract were enhanced after coating of the mucosal surfaces with the high-density barium suspension. Internal structures of the stomach and mucosal surfaces of the intestine were clearly identified. The barium contrast study was used to establish gastrointestinal transit times. The mean gastric emptying time was 11 +/- 4.27 (SEM) min and mean intestinal transit time was 5 +/- 0.75 (SEM) h. Image intensification fluoroscopy was used to observe patterns of small intestinal motility and to establish small intestinal contraction rates. Prominent cluster of circular contractions were primarily observed in the jejunum, and wave-type peristalsis was primarily observed in the duodenum. Isolated circular contractions and weak segmentation patterns were observed in the ileum. The mean contraction rate per minute was observed to be 14 +/- 2.12 (SEM) in the jejunum. We propose that under the conditions of the current study, radiographic investigation of gastrointestinal function in rats is a feasible and inexpensive procedure.
本研究首次报告了在大鼠腹部X线平片上对解剖结构的识别，以及对正常大鼠胃肠道进行详细的钡剂造影研究。大鼠（49至112日龄）通过肌肉注射0.71mg盐酸氯胺酮/克体重与0.5mg马来酸乙酰丙嗪的组合进行化学约束。镇静后，通过胃管以0.02ml/克体重的剂量给予36%（重量/体积）的微粉化硫酸钡悬浮液。用高密度钡悬浮液覆盖粘膜表面后，胃肠道的X线特征得到增强。胃的内部结构和肠道的粘膜表面清晰可见。钡剂造影研究用于确定胃肠道转运时间。平均胃排空时间为11±4.27（标准误）分钟，平均肠道转运时间为5±0.75（标准误）小时。采用影像增强荧光透视法观察小肠蠕动模式并确定小肠收缩率。主要在空肠观察到明显的环形收缩簇，主要在十二指肠观察到波状蠕动。在回肠观察到孤立的环形收缩和微弱的分段模式。空肠每分钟的平均收缩率为14±2.12（标准误）。我们认为，在本研究条件下，对大鼠胃肠道功能进行X线检查是一种可行且廉价的方法。
