Creti R, Ceccarelli E, Bocchetta M, Sanangelantoni A M, Tiboni O, Palm P, Cammarano P
Istituto Pasteur-Fondazione Cenci-Bolognetti, Dipt. Biopatologia Umana, Università di Roma I, Policlinico Umberto I., Italy.
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3255-9. doi: 10.1073/pnas.91.8.3255.
The EF-2 coding genes of the Archaea Pyrococcus woesei and Desulfurococcus mobilis were cloned and sequenced. Global phylogenies were inferred by alternative tree-making methods from available EF-2(G) sequence data and contrasted with phylogenies constructed from the more conserved but shorter EF-1 alpha(Tu) sequences. Both the monophyly (sensu Hennig) of Archaea and their subdivision into the kingdoms Crenarchaeota and Euryarchaeota are consistently inferred by analysis of EF-2(G) sequences, usually at a high bootstrap confidence level. In contrast, EF-1 alpha(Tu) phylogenies tend to be inconsistent with one another and show low bootstrap confidence levels. While evolutionary distance and DNA maximum parsimony analyses of EF-1 alpha(Tu) sequences do show archaeal monophyly, protein parsimony and DNA maximum-likelihood analyses of these data do not. In no case, however, do any of the tree topologies inferred from EF-1 alpha(Tu) sequence analyses receive significant bootstrap support.
嗜热栖热球菌（Pyrococcus woesei）和运动脱硫球菌（Desulfurococcus mobilis）的EF-2编码基因被克隆并测序。通过替代的建树方法，从可用的EF-2（G）序列数据推断出全局系统发育，并与由更保守但更短的EF-1α（Tu）序列构建的系统发育进行对比。通过对EF-2（G）序列的分析，通常在高自展置信水平下，始终推断出古菌的单系性（按照亨尼希的意义）及其细分为泉古菌门（Crenarchaeota）和广古菌门（Euryarchaeota）。相比之下，EF-1α（Tu）系统发育往往彼此不一致，并且显示出自展置信水平较低。虽然对EF-1α（Tu）序列的进化距离和DNA最大简约分析确实显示出古菌的单系性，但对这些数据的蛋白质简约和DNA最大似然分析则不然。然而，在任何情况下，从EF-1α（Tu）序列分析推断出的任何树形拓扑都没有得到显著的自展支持。
