Wyatt C R, Madruga C, Cluff C, Parish S, Hamilton M J, Goff W, Davis W C
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Vet Immunol Immunopathol. 1994 Mar;40(3):187-99. doi: 10.1016/0165-2427(94)90019-1.
A panel of monoclonal antibodies to bovine leukocyte differentiation molecules was used to evaluate peripheral blood and splenic lymphocytes from cattle of various ages. The major population of peripheral blood lymphocytes from neonatal calves was gamma delta T-cell receptor (TCR1) positive, as determined by TCR1-N12 expression. TCR1-N12+ lymphocytes were decreased in number in older calves, and were lowest in adult cattle. The major subpopulation of TCR1-N12+ cells from peripheral blood coexpressed WC1, but not BoCD2. A small subpopulation of peripheral blood TCR1-N12+ cells from cattle of all ages coexpressed BoCD2, but not WC1. The TCR1-N12+ BoCD2+ lymphocytes made up the largest TCR1-N12+ lymphocyte subpopulation in spleens of both calves and adults. The TCR1-N12+WC1+ splenic lymphocytes were present as a small population. The data indicate that two subpopulations of TCR1+ lymphocytes are present in cattle of all ages. These two subpopulations are differentially distributed between blood and spleen, with TCR1-N12+WC1+ lymphocytes predominating in blood, and TCR1-N12+BoCD2+ cells predominating in spleen.
一组针对牛白细胞分化分子的单克隆抗体被用于评估不同年龄段牛的外周血淋巴细胞和脾淋巴细胞。通过TCR1-N12表达测定，新生犊牛外周血淋巴细胞的主要群体为γδT细胞受体（TCR1）阳性。在年龄较大的犊牛中，TCR1-N12+淋巴细胞数量减少，在成年牛中最少。外周血中TCR1-N12+细胞的主要亚群共表达WC1，但不表达BoCD2。所有年龄段牛的外周血中一小部分TCR1-N12+细胞共表达BoCD2，但不表达WC1。TCR1-N12+BoCD2+淋巴细胞在犊牛和成年牛脾脏中构成最大的TCR1-N12+淋巴细胞亚群。TCR1-N12+WC1+脾淋巴细胞数量较少。数据表明，所有年龄段的牛中都存在两个TCR1+淋巴细胞亚群。这两个亚群在血液和脾脏中的分布不同，TCR1-N12+WC1+淋巴细胞在血液中占主导，而TCR1-N12+BoCD2+细胞在脾脏中占主导。
