Wyatt C R, Brackett E J, Perryman L E, Davis W C
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040, USA.
Vet Immunol Immunopathol. 1996 Jun 15;52(1-2):91-103. doi: 10.1016/0165-2427(95)05535-5.
Ileal intraepithelial and lamina propria lymphocytes from newborn, 1.5-week-old, and 3-week-old calves were compared to determine to what extent the mucosa becomes populated after birth. Single and dual fluorescence flow cytometry were used with monoclonal antibodies to bovine (Bo) CD molecules to identify lymphocyte subpopulations. Few ileal mucosal lymphocytes were present in calves at birth. However, by 1.5 weeks of age, the villi were populated with large numbers of lymphocytes, and by 3 weeks of age, the numbers had increased further. These included a prominent subpopulation of gamma delta T cells. Several subsets of gamma delta T cells populated ileal mucosa after birth. The predominant subset coexpressed BoCD2, and a smaller subset coexpressed BoCD8. WC1+ gamma delta T cells comprised the smallest subset. All gamma delta T cell subsets coexpressed ACT2, a molecule expressed on activated WC1+ and WC1- gamma delta T cells from cattle.
对新生犊牛、1.5周龄犊牛和3周龄犊牛的回肠上皮内淋巴细胞和固有层淋巴细胞进行比较，以确定出生后黏膜的淋巴细胞定植程度。使用单荧光和双荧光流式细胞术，结合针对牛（Bo）CD分子的单克隆抗体，来鉴定淋巴细胞亚群。出生时犊牛回肠黏膜淋巴细胞很少。然而，到1.5周龄时，绒毛上布满了大量淋巴细胞，到3周龄时，数量进一步增加。其中包括一个显著的γδT细胞亚群。出生后有几个γδT细胞亚群定植于回肠黏膜。主要亚群共表达BoCD2，较小的亚群共表达BoCD8。WC1+γδT细胞组成最小的亚群。所有γδT细胞亚群均共表达ACT2，ACT2是一种在牛的活化WC1+和WC1-γδT细胞上表达的分子。
