Sato K, Sato F
Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242-1181.
Am J Physiol. 1994 Mar;266(3 Pt 2):R950-9. doi: 10.1152/ajpregu.1994.266.3.R950.
We wished to establish the presence of interleukin-1 (IL-1) in human sweat (5) and clarify its origin and mechanism of secretion. IL-1 alpha concentration ([IL-1 alpha]) in clean sweat from the back increased with the sweat rate, plateauing at the maximal sweat rate ([IL-1 alpha]max). The mean [IL-1 alpha]max was 545 pg/ml (n = 17) for men and 1,324 pg/ml for women in back sweat. The mean [IL-1 alpha]max for axillary sweat in men was 1,568 (n = 6). Palmar sweat was 9.2 ng/ml (n = 5) for IL-1 alpha and 7.9 ng/ml for IL-1 beta. [IL-1 alpha]max decreased to one-third that of the first sweat test, when second sauna sweat tests were conducted after 2 h of continuous sweating on the same day. Western blot analysis of the purified sweat IL-1 alpha fraction revealed bands at 17, 29, and 33 kDa. Immunoreactive IL-1 alpha was localized mainly in the secretory coil lumen, intercellular canaliculi, cytoplasm, mitochondria, and near plasma membranes. Polymerase chain reaction revealed the presence of IL-1 alpha mRNA in the sweat gland and in cultured human eccrine secretory coil cells. Both sweat IL-1 alpha and human recombinant IL-1 alpha at 500 pg/ml strongly stimulated interleukin-6 and interleukin-8 production in cultured fibroblasts. We conclude that the IL-1 alpha-like immunoreactive substance in sweat is IL-1 alpha itself, is derived from the sweat gland, and is biologically active at concentrations normally present in fresh sweat.
我们希望确定白细胞介素-1（IL-1）在人体汗液中的存在情况，并阐明其来源及分泌机制。背部清洁汗液中IL-1α浓度（[IL-1α]）随出汗速率增加而升高，在最大出汗速率（[IL-1α]max）时达到平稳状态。男性背部汗液中[IL-1α]max的平均值为545 pg/ml（n = 17），女性为1324 pg/ml。男性腋窝汗液中[IL-1α]max的平均值为1568（n = 6）。手掌汗液中IL-1α为9.2 ng/ml（n = 5），IL-1β为7.9 ng/ml。在同一天持续出汗2小时后进行第二次桑拿汗液测试时，[IL-1α]max降至首次汗液测试的三分之一。对纯化的汗液IL-1α组分进行蛋白质印迹分析，结果显示在17 kDa、29 kDa和33 kDa处出现条带。免疫反应性IL-1α主要定位于分泌蟠管腔、细胞间小管、细胞质、线粒体及靠近质膜处。聚合酶链反应显示在汗腺及培养的人外泌汗腺分泌蟠管细胞中存在IL-1α mRNA。汗液IL-1α和500 pg/ml的重组人IL-1α均能强烈刺激培养的成纤维细胞产生白细胞介素-6和白细胞介素-8。我们得出结论，汗液中IL-1α样免疫反应性物质即为IL-1α本身，它来源于汗腺，且在新鲜汗液中通常存在的浓度下具有生物活性。
