Rosengren A, Johansson B R, Thomsen P, Ericson L E
Department of Anatomy, University of Göteborg, Sweden.
Biomaterials. 1994 Jan;15(1):17-24. doi: 10.1016/0142-9612(94)90190-2.
A method allowing immunolocalization of macromolecules surrounding biomaterial implants has been developed. Plugs of pure titanium were inserted into the abdominal wall of rats. One week after implantation plugs with adjacent tissue were gently fixed and cryoprotected before being rapidly frozen in LN2-cooled propane, followed by cryosubstitution, using methanol, and low temperature infiltration and UV curing of the methacrylate LR-Gold. Before sectioning, bulk metal was removed electrochemically, leaving the surface oxide intact, appearing in sections as a dense line in contact with the tissue. Postembedding immunolocalization for rat albumin, IgG, C3c, fibrinogen and fibronectin was performed at the light and electron microscopic levels. Our observations indicate that 1 wk after implant insertion, a network of fibrin and fibronectin decorates the tissue surface that faces the implant, but without any obvious concentration to the implant surface.
已开发出一种可对生物材料植入物周围大分子进行免疫定位的方法。将纯钛栓子植入大鼠腹壁。植入一周后，将带有相邻组织的栓子轻轻固定并进行冷冻保护，然后在液氮冷却的丙烷中快速冷冻，接着进行冷冻置换（使用甲醇）以及甲基丙烯酸酯LR-金的低温渗透和紫外线固化。在切片之前，通过电化学方法去除大块金属，使表面氧化物保持完整，在切片中呈现为与组织接触的致密线条。在光镜和电镜水平上对大鼠白蛋白、IgG、C3c、纤维蛋白原和纤连蛋白进行包埋后免疫定位。我们的观察结果表明，植入栓子1周后，纤维蛋白和纤连蛋白网络覆盖着面向植入物的组织表面，但在植入物表面没有明显的聚集。
