Rosengren A, Johansson B R, Danielsen N, Thomsen P, Ericson L E
Department of Physiology and Neuroscience, Lund University, Sweden.
Biomaterials. 1996 Sep;17(18):1779-86. doi: 10.1016/0142-9612(95)00358-4.
Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.
利用最近开发的一种方法，研究了细胞外蛋白质（白蛋白、纤维蛋白原、纤连蛋白、I型胶原蛋白和IgG）在植入物与软组织界面区域的时间依赖性分布。将商业纯（c.p.）钛和聚四氟乙烯（PTFE）植入物插入大鼠腹壁1、6和12周，随后进行轻度固定、冷冻保护、在液氮冷却的丙烷中快速冷冻、冷冻置换以及用甲基丙烯酸酯LR-金进行紫外线固化的低温浸润。在切片之前，通过电解溶解技术（电解抛光）去除大部分钛，而PTFE植入物则通过断裂技术去除。采用冷冻置换方法结合包埋后免疫组织化学进行光镜分析。所选蛋白质在植入物附近组织中的分布明显不同，并且在随访期间其分布发生了变化。每种蛋白质在钛和PTFE植入物周围的分布模式也存在差异。c.p.钛植入物的插入引发了许多方面类似于正常伤口愈合反应的炎症反应，而PTFE植入物则引起了更明显、持续时间更长的炎症。
