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源自流产布鲁氏菌的核糖体组分的免疫原活性。

The immunogenic activity of ribosomal fractions derived from Brucella abortus.

作者信息

Corbel M J

出版信息

J Hyg (Lond). 1976 Feb;76(1):65-74. doi: 10.1017/s0022172400054954.

DOI:10.1017/s0022172400054954
PMID:812900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129614/
Abstract

The immunizing activity of ribosome preparations derived from Brucella abortus strain 19 cells was examined in guinea-pigs and mice. After subcutaneous injections of Br. abortus ribosomes in Freund's incomplete adjuvant, both mice and guinea-pigs developed immunity to challenge by virulent Br. abortus 544 organisms which was at least as effective as the protection conferred by live strain 19 vaccine. Both mice and guinea-pigs also developed agglutinating and complement-fixing antibodies and delayed hypersensitivity to Br. Abortus antigens. Conversely, ribosome preparations elicited delayed hypersensitivity reactions on intracutaneous injection into guinea-pigs chronically infected with Br. abortus or Br. melitensis. On injection into rabbits, Br. abortus ribosomes incorporated in incomplete adjuvant induced high titres of agglutinins, complement fxing antibodies and precipitins for Br. abortus antigens. On immunochemical examination, the ribosome preparations were not grossly contaminated with antigens derived from the cell surface. They were chemically complex, however, and in addition to RNA contained numerous protein components identified by disk electrophoresis. The nature of the components responsible for conferring protection against Br. abortus was not determined.

摘要

对源自流产布鲁氏菌19菌株细胞的核糖体制剂的免疫活性在豚鼠和小鼠中进行了检测。在弗氏不完全佐剂中皮下注射流产布鲁氏菌核糖体后，小鼠和豚鼠均对强毒流产布鲁氏菌544菌株的攻击产生了免疫力，其效果至少与活19菌株疫苗提供的保护一样有效。小鼠和豚鼠还产生了凝集抗体和补体结合抗体，并对流产布鲁氏菌抗原产生了迟发型超敏反应。相反，核糖体制剂在皮内注射到长期感染流产布鲁氏菌或马尔他布鲁氏菌的豚鼠体内时会引发迟发型超敏反应。将流产布鲁氏菌核糖体掺入不完全佐剂中注射到兔子体内时，会诱导产生高滴度的针对流产布鲁氏菌抗原的凝集素、补体结合抗体和沉淀素。经免疫化学检测，核糖体制剂未被源自细胞表面的抗原严重污染。然而，它们化学组成复杂，除RNA外，还含有通过圆盘电泳鉴定的众多蛋白质成分。赋予针对流产布鲁氏菌保护作用的成分的性质尚未确定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/62f8c708b3d5/jhyg00064-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/066e1c485ede/jhyg00064-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/fe05b645f33e/jhyg00064-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/06202470c912/jhyg00064-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/85ae3af33f9f/jhyg00064-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/ddbf1509a946/jhyg00064-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/49c41946280b/jhyg00064-0080-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/dfa3ec19ac0e/jhyg00064-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/62f8c708b3d5/jhyg00064-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/066e1c485ede/jhyg00064-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/fe05b645f33e/jhyg00064-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/06202470c912/jhyg00064-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/85ae3af33f9f/jhyg00064-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/ddbf1509a946/jhyg00064-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/49c41946280b/jhyg00064-0080-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/dfa3ec19ac0e/jhyg00064-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0908/2129614/62f8c708b3d5/jhyg00064-0083-b.jpg

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本文引用的文献

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Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium.从鼠伤寒沙门氏菌中获得的核糖核酸制剂的免疫原性。
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IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS.从结核分枝杆菌获得的核糖体组分的免疫原活性
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Delayed-type hypersensitivity activity of the Brucella L7/L12 ribosomal protein depends on posttranslational modification.布鲁氏菌L7/L12核糖体蛋白的迟发型超敏反应活性取决于翻译后修饰。
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Brucella ribosomal protein L7/L12 is a major component in the antigenicity of brucellin INRA for delayed-type hypersensitivity in brucella-sensitized guinea pigs.布鲁氏菌核糖体蛋白L7/L12是布鲁氏菌素INRA抗原性的主要成分，该抗原可引起布鲁氏菌致敏豚鼠的迟发型超敏反应。
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Recombinant L7/L12 ribosomal protein and gamma-irradiated Brucella abortus induce a T-helper 1 subset response from murine CD4+ T cells.重组L7/L12核糖体蛋白和γ射线照射的流产布鲁氏菌可诱导小鼠CD4+ T细胞产生辅助性T细胞1亚群反应。
Immunology. 1994 Dec;83(4):659-64.
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Direct evidence for the involvement of capsular polysaccharide in the immunoprotective activity of Klebsiella pneumoniae ribosomal preparations.关于荚膜多糖参与肺炎克雷伯菌核糖体制剂免疫保护活性的直接证据。
Infect Immun. 1981 Jan;31(1):71-7. doi: 10.1128/iai.31.1.71-77.1981.
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Variability of protection in inbred mice induced by a ribosomal vaccine prepared from Salmonella typhimurium.由鼠伤寒沙门氏菌制备的核糖体疫苗诱导的近交系小鼠保护作用的变异性。
Infect Immun. 1976 Sep;14(3):652-9. doi: 10.1128/iai.14.3.652-659.1976.
J Exp Med. 1964 Jul 1;120(1):105-20. doi: 10.1084/jem.120.1.105.
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The chemical basis of the virulence of Brucella abortus. IV. Immunogenic products from Brucella abortus grown in vivo and in vitro.流产布鲁氏菌毒力的化学基础。IV. 体内和体外培养的流产布鲁氏菌的免疫原性产物。
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Antibacterial immunity induced by ribosomal vaccines.核糖体疫苗诱导的抗菌免疫
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