Lloyd D H, Sasiak A B, Kitson S, McEwan Jenkinson D, Elder H Y
Dermatology Unit, Royal Veterinary College, Herts, Royaume-Uni.
Rev Elev Med Vet Pays Trop. 1993;46(1-2):271-6.
The patterns of dermal inflammatory cell response to infection with Dermatophilosis congolensis were determined in mice and sheep from histological samples taken before and at intervals after topical application of infective zoospores to ether-swabbed skin. Neutrophils, eosinophils, basophils and mast cells were identified by histochemical staining. Temporal changes in the B cell, T cell, and MHC Class II+ dendritic cell populations form part of a separate report. The filamentous stages of the bacterium were observed in the stratum corneum of both species; in the sheep they were also found in the outer layers of the living epidermis. In both species, large numbers of neutrophils and some lymphocytes penetrated the epidermis and entered the infected surface region. Within the underlying dermis there was an accumulation of dendritic cells immediately below the infected epidermis and evidence of mast cell degranulation; the basophils and eosinophils did not appear to be actively involved. The striking difference between the two species was the duration of the infection and the associated response which, in the mouse, lasted about five days in comparison with over 21 days in the sheep. Neutrophil numbers in the mouse for example were elevated by 12 h and had peaked at 60 h after infection, while in the sheep they did not peak until about 120 h.
通过对用感染性游动孢子局部涂抹于经乙醚擦拭的皮肤后不同时间点采集的小鼠和绵羊的组织学样本进行分析，确定了刚果嗜皮菌感染引起的皮肤炎症细胞反应模式。通过组织化学染色鉴定中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞。B细胞、T细胞和MHC II类+树突状细胞群体的时间变化构成了一份单独报告的一部分。在两种动物的角质层中均观察到该细菌的丝状阶段；在绵羊中，在活表皮的外层也发现了丝状阶段。在两种动物中，大量中性粒细胞和一些淋巴细胞穿透表皮并进入感染表面区域。在下层真皮中，感染表皮下方立即有树突状细胞聚集，并有肥大细胞脱颗粒的迹象；嗜碱性粒细胞和嗜酸性粒细胞似乎未积极参与。两种动物之间的显著差异在于感染持续时间及相关反应，例如，小鼠的感染持续约5天，而绵羊的感染持续超过21天。例如，小鼠的中性粒细胞数量在感染后12小时升高，在60小时达到峰值，而绵羊的中性粒细胞直到约120小时才达到峰值。
