Poermadjaja B, Ambrose N, Walker A, Morrow A
Centre for Tropical Veterinary Medicine, Roslin, Midiothian, Ecosse, Grande-Bretagne.
Rev Elev Med Vet Pays Trop. 1993;46(1-2):277-82.
The cellular infiltrate into the dermis in dermatophilosis lesions is composed of a range of cell types. The aim of this study was to establish if the composition of the cellular infiltrate in chronic lesions was different from that in healing lesions. Experimental Dermatophilus congolensis infections of sheep were used to study the sequential changes in cell types during the course of chronic and acute infections. Infestations of adult Amblyomma variegatum ticks were used to produce chronic lesions on infected sheep, infections of tick-free sheep provided acute lesions. Histopathology and immunohistochemistry were used to stain and label cell types in the dermis of infection sites. Neutrophils dominated the early response and were present in larger numbers in chronic lesions. Plasma cells were present in both types of lesion, however they persisted in chronic lesions but disappeared from the skin at acute lesion sites after the lesions had resolved. There were 2-3 times as many mononuclear cells in chronic than acute lesions from as early as 4 days post infection and these cells persisted in the chronic lesions. In the chronic lesions the mononuclear cell population was composed of T-helper and T-cytotoxic/suppressor lymphocytes in equal proportions whereas in acute lesions at 14 days post infection, when lesion resolution is underway, there were greater numbers of T-helper cells than T-cytotoxic/suppressor cells.
皮肤嗜皮菌病病变中浸润至真皮的细胞由多种细胞类型组成。本研究的目的是确定慢性病变中细胞浸润的组成是否与愈合病变中的不同。利用绵羊的实验性刚果嗜皮菌感染来研究慢性和急性感染过程中细胞类型的序贯变化。使用成年杂色花蜱侵染来在感染绵羊身上产生慢性病变，未感染蜱的绵羊感染则产生急性病变。采用组织病理学和免疫组织化学方法对感染部位真皮中的细胞类型进行染色和标记。中性粒细胞在早期反应中占主导地位，且在慢性病变中的数量更多。两种病变中均有浆细胞存在，然而它们在慢性病变中持续存在，而在急性病变部位，病变消退后浆细胞从皮肤中消失。早在感染后4天起，慢性病变中的单核细胞数量就是急性病变中的2至3倍，且这些细胞在慢性病变中持续存在。在慢性病变中，单核细胞群体由等量的辅助性T淋巴细胞和细胞毒性/抑制性T淋巴细胞组成，而在感染后14天的急性病变中，当病变正在消退时，辅助性T细胞的数量多于细胞毒性/抑制性T细胞。
