Wu M Y, Chao K H, Chen S U, Chen H F, Yang Y S, Huang S C, Ho H N
Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Am J Reprod Immunol. 1996 Jun;35(6):510-6. doi: 10.1111/j.1600-0897.1996.tb00050.x.
Our previous study reported that peritoneal natural killer (NK) cytotoxicity and CD3+CD25+ lymphocyte subpopulation were suppressed in women with advanced endometriosis. Whether these phenomena are general for all stages of endometriosis and whether these alterations could be restored by long-term use of gonadotropin releasing hormone agonist (GnRHa) are further tested in this study.
Lymphocyte subpopulations (B cells, NK cells, T cells, and T-cell activation markers such as CD69, HLA-DR, and CD25) and NK cell cytotoxicity of peripheral blood and peritoneal fluid by dual-color flow cytometry and 51Cr release assay in 30 cases of endometriosis were compared with those in 26 controls. We also compared these changes before and after 6-month treatment with GnRHa for advanced endometriosis.
Compared with the controls, only those women with advanced endometriosis showed lower NK cytotoxicity in peritoneal fluid mononuclear cells (PFMC). The CD3+CD69+ lymphocyte subpopulation decreased in peripheral blood mononuclear cells (PBMC) of advanced endometriosis, while the CD3+CD25+ lymphocyte subpopulation decreased in both PBMC and PFMC of mild and advanced endometriosis. After GnRHa treatment, the CD3+CD69+ lymphocyte subpopulation increased in both PBMC and PFMC and the CD3+CD25+ lymphocyte subpopulation increased in PFMC, but not in PBMC.
Impaired local immunological function in the PF of endometriosis was confirmed by this study and the impairments could be restored after long-term GnRHa therapy.
我们之前的研究报道，晚期子宫内膜异位症女性的腹膜自然杀伤（NK）细胞毒性及CD3⁺CD25⁺淋巴细胞亚群受到抑制。本研究进一步检验这些现象是否在子宫内膜异位症的所有阶段都普遍存在，以及这些改变能否通过长期使用促性腺激素释放激素激动剂（GnRHa）得以恢复。
采用双色流式细胞术和⁵¹Cr释放试验，比较30例子宫内膜异位症患者外周血和腹腔液中的淋巴细胞亚群（B细胞、NK细胞、T细胞以及T细胞活化标志物如CD69、HLA - DR和CD25）和NK细胞毒性，与26例对照者进行对比。我们还比较了晚期子宫内膜异位症患者在GnRHa治疗6个月前后的这些变化。
与对照组相比，仅晚期子宫内膜异位症女性的腹腔液单核细胞（PFMC）中的NK细胞毒性较低。晚期子宫内膜异位症患者外周血单核细胞（PBMC）中的CD3⁺CD69⁺淋巴细胞亚群减少，而轻度和晚期子宫内膜异位症患者的PBMC和PFMC中的CD3⁺CD25⁺淋巴细胞亚群均减少。GnRHa治疗后，PBMC和PFMC中的CD3⁺CD69⁺淋巴细胞亚群均增加，PFMC中的CD3⁺CD25⁺淋巴细胞亚群增加，但PBMC中未增加。
本研究证实了子宫内膜异位症患者腹腔液中局部免疫功能受损，且长期GnRHa治疗后这些损伤可以恢复。
