Milman N, Graudal N, Juul-Jørgensen B, Bentzon M W
Department of Medicine Y, Gentofte Hospital, Copenhagen, Denmark.
Eur J Clin Chem Clin Biochem. 1994 Jan;32(1):41-2.
A commercial ferritin kit (Amersham Ferritin RIA (A)) was calibrated using the WHO human liver ferritin international standard 80/602 (W). The reconstituted WHO freeze-dried standard was diluted to obtain five concentration levels ranging from 10-800 micrograms/l. Logarithmic transformation of the values was performed in order to stabilize the variance, yielding the regression equation: logA = 0.0235 + 1.0022 logW. The slope of the regression line (being very close to, and not significantly different from, one) was set to one, and the relation between the untransformed values then became a proportionality: A = 1.067 x W. A WHO standard ferritin value of 15 micrograms/l (often used as cut-off value for absent iron reserves) and of 30 mu/l (often used as threshold value for the presence of stainable marrow haemosiderin iron) yielded calculated Amersham Ferritin RIA values of 16.0 micrograms/l and 32.0 micrograms/l.
使用世界卫生组织人肝铁蛋白国际标准品80/602（W）对一种商用铁蛋白试剂盒（Amersham铁蛋白放射免疫分析试剂盒（A））进行校准。将重构后的世界卫生组织冻干标准品进行稀释，以获得浓度范围为10 - 800微克/升的五个浓度水平。对这些值进行对数转换以稳定方差，得到回归方程：logA = 0.0235 + 1.0022 logW。将回归线的斜率（非常接近1且与1无显著差异）设为1，那么未转换值之间的关系就变成了比例关系：A = 1.067×W。世界卫生组织标准铁蛋白值15微克/升（常被用作无铁储备的临界值）和30微克/升（常被用作可染色骨髓含铁血黄素铁存在的阈值）得出的Amersham铁蛋白放射免疫分析计算值分别为16.0微克/升和32.0微克/升。
