Ho P C, Yeung W S, Chan Y F, So W W, Chan S T
Int J Fertil Menopausal Stud. 1994 Jan-Feb;39(1):14-9.
To study the factors affecting the incidence of polyploidy in an assisted reproduction program.
Retrospective analysis.
University-based subfertility clinic.
Subfertile patients were treated in 163 treatment cycles of in vitro fertilization (IVF) or pronuclear stage tubal transfer (PROST). The relationship between the incidence of polyspermy and the indication of subfertility, the stimulation protocol, the oocyte, and the semen parameters were analyzed.
Eighty-nine of the 1,149 oocytes were polyploid (7.8%). The incidence of polyploidy was not affected by the indication for IVF, the age of the patients, the stimulation protocol, the maturity of oocytes as assessed by the appearance of the cumulus, the sperm concentration, the number of sperm inseminated, the serum estradiol level, and the number of oocytes retrieved. The incidence was increased when: (1) the serum estradiol fell before the administration of human chorionic gonadotrophin; (2) the oocyte retrieval-insemination interval was 6 hours or more; (3) the sperm motility was 70% or more; (4) the percentage of sperm with normal morphology was 50% or more.
The incidence of polyploidy was affected by the serum estradiol pattern, the preinsemination interval, sperm motility, and percentage of morphologically normal sperm.
研究辅助生殖项目中影响多倍体发生率的因素。
回顾性分析。
大学附属不孕不育诊所。
对163个体外受精（IVF）或原核期输卵管内移植（PROST）治疗周期中的不孕不育患者进行治疗。分析多精受精发生率与不孕不育指征、刺激方案、卵母细胞及精液参数之间的关系。
1149个卵母细胞中有89个为多倍体（7.8%）。多倍体发生率不受IVF指征、患者年龄、刺激方案、根据卵丘外观评估的卵母细胞成熟度、精子浓度、授精精子数量、血清雌二醇水平及获取的卵母细胞数量的影响。在以下情况发生率会增加：（1）在注射人绒毛膜促性腺激素前血清雌二醇水平下降；（2）卵母细胞获取 - 授精间隔为6小时或更长；（3）精子活力为70%或更高；（4）形态正常精子的百分比为50%或更高。
多倍体发生率受血清雌二醇模式、授精前间隔、精子活力及形态正常精子百分比的影响。
