利用随机扩增多态性DNA（RAPD）标记对以色列野生大麦种群的遗传变异进行检测与分析。

Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers.

作者信息

Dawson I K, Chalmers K J, Waugh R, Powell W

机构信息

Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee, UK.

出版信息

Mol Ecol. 1993 Jun;2(3):151-9. doi: 10.1111/j.1365-294x.1993.tb00104.x.

DOI:10.1111/j.1365-294x.1993.tb00104.x

PMID:8167849

Abstract

Randomly amplified polymorphic DNA (RAPD) markers were used to analyse genetic diversity within and between Hordeum spontaneum populations sampled from Israel. Nei's index of genetic differentiation was used to partition diversity into within and between population components. Fifty-seven per cent of the variation detected was partitioned within 10 H. spontaneum populations. Using principal component and multiple regression analysis, part of the variation detected between populations was seen to be associated with certain ecogeographical factors. Fifty-eight per cent of the distribution of the phenotypic frequencies of three RAPD phenotypes detected using a single primer in 20 H. spontaneum populations could be accounted for by four ecogeographical variables, suggesting adaptive variation at certain RAPD loci.

摘要

利用随机扩增多态性DNA（RAPD）标记来分析从以色列采集的野生大麦种群内部和种群之间的遗传多样性。采用内氏遗传分化指数将多样性划分为种群内和种群间的组分。检测到的变异中有57%分布在10个野生大麦种群内。通过主成分分析和多元回归分析发现，种群间检测到的部分变异与某些生态地理因素有关。在20个野生大麦种群中，使用单个引物检测到的三种RAPD表型的表型频率分布中有58%可由四个生态地理变量来解释，这表明在某些RAPD位点存在适应性变异。