Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, Scotland.
Plant Cell Rep. 1996 Jan;15(5):337-41. doi: 10.1007/BF00232367.
The phenetic relationships between 18 Coffea accessions representing 11 of the most important Coffea species employed in current breeding programmes were examined using RAPD markers and chloroplast and mitochondrial genome specific sequence tagged sites (STS). Estimates of variability based on the number of shared RAPD amplification products placed the species into three distinct groups which were consistent with derived chloroplast DNA phenotypes, the geographical origins of the species and previous studies based on morphological characteristics and RFLPs. C. eugenioides (2n = 2x = 22) exhibited the greatest similarity to the cultivated C. arabica (2n = 4x = 44) and may represent its maternal progenitor. The results are discussed in the context of strategies for Coffea improvement.
利用 RAPD 标记和叶绿体及线粒体基因组特异序列标记位点（STS），研究了代表当前育种计划中 11 个最重要咖啡种的 18 个咖啡栽培品种间的表型关系。基于共享 RAPD 扩增产物数量的变异性估计，将这些种分为三个不同的组，这与衍生的叶绿体 DNA 表型、种的地理起源以及以前基于形态特征和 RFLP 的研究一致。C. eugenioides（2n=2x=22）与栽培的 C. arabica（2n=4x=44）表现出最大的相似性，可能代表其母本祖先。讨论结果时考虑了咖啡改良的策略。
