Bratthauer G L, Cardiff R D, Fanning T G
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
Cancer. 1994 May 1;73(9):2333-6. doi: 10.1002/1097-0142(19940501)73:9<2333::aid-cncr2820730915>3.0.co;2-4.
Several diseases have been linked to the insertion of human LINE-1 retrotransposons (L1Hs) into structural genes. Recently, the element has been shown to be expressed in a variety of adult and pediatric germ cell cancers, leading to speculation that L1Hs-induced insertion mutations may play a role in the etiology of some neoplasias.
An L1Hs-encoded protein (p40) was assayed in breast cancer cell lines by Western blotting and in solid tumors by immunohistochemical staining and Western blotting.
L1Hs retrotransposons are expressed in a significant number of human breast cancers: expression was detected in 7 of 8 malignant cell lines and in 9 of 12 primary infiltrating ductal carcinomas. No expression was detected in two nonmalignant breast epithelial cell lines, five malignant B- or T-cell lines, tissue from a normal breast, a primary breast sarcoma, or a primary medullary carcinoma of the breast.
These results raise the possibility that L1Hs expression may contribute to the origin or progression of some breast cancers.
多种疾病与人类LINE-1逆转录转座子（L1Hs）插入结构基因有关。最近，已证明该元件在多种成人和儿童生殖细胞癌中表达，这引发了人们的猜测，即L1Hs诱导的插入突变可能在某些肿瘤的病因学中起作用。
通过蛋白质免疫印迹法在乳腺癌细胞系中检测L1Hs编码的蛋白（p40），并通过免疫组织化学染色和蛋白质免疫印迹法在实体瘤中进行检测。
L1Hs逆转录转座子在大量人类乳腺癌中表达：在8个恶性细胞系中的7个以及12个原发性浸润性导管癌中的9个中检测到表达。在两个非恶性乳腺上皮细胞系、五个恶性B或T细胞系、正常乳腺组织、原发性乳腺肉瘤或原发性乳腺髓样癌中未检测到表达。
这些结果增加了L1Hs表达可能促成某些乳腺癌的发生或进展的可能性。
