Suzuki K, Bakaletz L O
Department of Otolaryngology, Ohio State University College of Medicine, Columbus 43210.
Infect Immun. 1994 May;62(5):1710-8. doi: 10.1128/iai.62.5.1710-1718.1994.
We recently reported the development of a chinchilla model of experimental otitis media (OM) that uses a pediatric clinical isolate of adenovirus type 1 (4) and in which an active infection with the wild-type strain was demonstrated. To expand upon these findings, this study was designed to determine whether we could demonstrate adenovirus infection-induced predisposition to bacterial OM in the chinchilla, as has been shown in human epidemiological studies (D. A. Clements, F. W. Henderson, and E. C. Neebe, p. 27-29, in D. J. Lim, C. D. Bluestone, J. O. Klein, D. J. Nelson, and P. L. Ogra, ed., Proceedings of the Fifth International Symposium on Recent Advances in Otitis Media, 1993; F. W. Henderson, A. M. Collier, M. A. Sanyai, et al., N. Engl. J. Med. 306:1377-1383, 1982). In addition, we were interested in determining whether altering the order of pathogen acquisition would further affect the outcome of disease incidence and severity. Toward this end, cohorts of chinchillas were inoculated intranasally with a strain of nontypeable Haemophilus influenzae (NTHi) (86-028NP) which colonizes the chinchilla nasopharynx but does not consistently induce culture-positive OM when inoculated intranasally (L. O. Bakaletz, T. M. Hoepf, D. J. Lim, and B. Tallan, Abstr. 90th Annu. Meet. Am. Soc. Microbiol. 1990, abstr. B-66, p. 37, 1990), adenovirus type 1 and then inoculated 7 days later with NTHi, NTHi and then inoculated 7 days later with adenovirus type 1, or both pathogens concurrently. All cohorts were observed over a 35-day period and assessed for incidence and severity of OM by several methodologies. The data collectively indicated that all animals receiving both pathogens developed OM of greater severity than those receiving only a single agent. Adenovirus inoculation followed 7 days later by NTHi inoculation was the order of pathogen acquisition which induced the most prolonged presence of NTHi in both the nasopharynx and the middle ear, the most severe tympanic membrane inflammation overall, and the most significant damage to and altered function of both middle ear and eustachian tube mucosae.
我们最近报道了一种实验性中耳炎（OM）的栗鼠模型的建立，该模型使用1型腺病毒的儿科临床分离株（4），并证实了野生型菌株的活跃感染。为了扩展这些发现，本研究旨在确定我们是否能够在栗鼠中证明腺病毒感染诱导的细菌性中耳炎易感性，正如人类流行病学研究中所显示的那样（D.A.克莱门茨、F.W.亨德森和E.C.尼贝，第27 - 29页，载于D.J.林、C.D.布卢斯托、J.O.克莱因、D.J.纳尔逊和P.L.奥格拉编，《中耳炎最新进展第五届国际研讨会论文集》，1993年；F.W.亨德森、A.M.科利尔、M.A.桑亚伊等人，《新英格兰医学杂志》306:1377 - 1383，1982年）。此外，我们有兴趣确定改变病原体获取顺序是否会进一步影响疾病的发病率和严重程度。为此，将几组栗鼠经鼻接种一株不可分型流感嗜血杆菌（NTHi）（86 - 028NP），该菌株定殖于栗鼠鼻咽部，但经鼻接种时并不总是能诱导培养阳性的中耳炎（L.O.巴卡莱茨、T.M.赫普夫、D.J.林和B.塔兰，《美国微生物学会第90届年会摘要》，1990年，摘要B - 66，第37页，1990年），先接种1型腺病毒，7天后再接种NTHi，先接种NTHi，7天后再接种1型腺病毒，或同时接种两种病原体。所有组在35天内进行观察，并通过几种方法评估中耳炎的发病率和严重程度。总体数据表明，所有接受两种病原体的动物所患中耳炎的严重程度均高于仅接受单一病原体的动物。先接种腺病毒7天后再接种NTHi是病原体获取顺序，这种顺序导致NTHi在鼻咽部和中耳中存在的时间最长，总体鼓膜炎症最严重，中耳和咽鼓管黏膜的损伤和功能改变最显著。
