Weber T, Turner R W, Frye S, Ruf B, Haas J, Schielke E, Pohle H D, Lüke W, Lüer W, Felgenhauer K
Neurologische Klinik und Poliklinik, Georg-August-Universität, Göttingen, Germany.
J Infect Dis. 1994 May;169(5):1138-41. doi: 10.1093/infdis/169.5.1138.
Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF) samples from 28 patients with progressive multifocal leukoencephalopathy (PML) were analyzed. As controls, 116 samples were evaluated from 82 human immunodeficiency virus type 1 (HIV-1)-infected patients and 1 HIV-1-negative patient. Of the HIV-1-positive patients, 23 had cerebral toxoplasmosis, 10 had HIV leukoencephalopathy, and 49 had other neurologic complications. Detection of JC virus (JCV) DNA in CSF was increased 10-fold by the addition of carrier DNA before phenol-chloroform-isoamyl alcohol extraction. The primer pair JC 26/29, from the VP1/large T region, had a limit of detection of 10(5) JCV DNA molecules/100 microL. The primer pair JC 36/39, located in the large T gene region, had a 100-fold lower limit of detection. With JC 26/29, the sensitivity was 43% (12/28) and specificity was 100%. Using JC 36/39, sensitivity increased to 82% (23/28), and false-positive results were not observed. Diagnosis of PML is greatly aided by PCR analysis of CSF.
采用聚合酶链反应（PCR）对28例进行性多灶性白质脑病（PML）患者的34份脑脊液（CSF）样本进行了分析。作为对照，对82例1型人类免疫缺陷病毒（HIV-1）感染患者和1例HIV-1阴性患者的116份样本进行了评估。在HIV-1阳性患者中，23例患有脑弓形虫病，10例患有HIV白质脑病，49例患有其他神经系统并发症。在酚-氯仿-异戊醇提取前加入载体DNA，脑脊液中JC病毒（JCV）DNA的检测量增加了10倍。来自VP1/大T区域的引物对JC 26/29的检测限为10(5)个JCV DNA分子/100微升。位于大T基因区域的引物对JC 36/39的检测限低100倍。使用JC 26/29时，敏感性为43%（12/28），特异性为100%。使用JC 36/39时，敏感性提高到82%（23/28），未观察到假阳性结果。脑脊液的PCR分析对PML的诊断有很大帮助。
