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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Cuthbert A W, Shum W K

J Physiol. 1976 Mar;255(3):605-18. doi: 10.1113/jphysiol.1976.sp011298.

Sodium entry sites in the membranes of isolated epithelial cells prepared from bladders of toads (Bufo marinus) have been labelled with amiloride. The number of binding sites remained constant in suspensions for up to 100 hr. 2. In the presence of a protein synthesis inhibitor (cycloheximide, 0-5 mug/ml.) there was a decline in the density of binding sites was approximately exponential. Regression analysis gave a half-life of approximately 60 hr. 3. Aldosterone (5 X 10(-8) M) caused a significant (P less than 0-001) increase (50%) in the density of amiloride binding sites. Cells which had been treated with aldosterone had populations of binding sites which declined, in the presence of cycloheximide, at rates indistinguishable from those of untreated cells.

从蟾蜍（海蟾蜍）膀胱制备的分离上皮细胞膜中的钠进入位点已用氨氯吡脒进行标记。在悬浮液中，结合位点的数量在长达100小时内保持恒定。2. 在存在蛋白质合成抑制剂（环己酰亚胺，0 - 5微克/毫升）的情况下，结合位点的密度下降，大致呈指数关系。回归分析得出半衰期约为60小时。3. 醛固酮（5×10⁻⁸ M）使氨氯吡脒结合位点的密度显著增加（P＜0.001）（增加50%）。用醛固酮处理过的细胞，其结合位点群体在环己酰亚胺存在的情况下，下降速率与未处理细胞的速率无明显差异。

Cuthbert A W, Shum W K

J Physiol. 1976 Mar;255(3):605-18. doi: 10.1113/jphysiol.1976.sp011298.

Sodium entry sites in the membranes of isolated epithelial cells prepared from bladders of toads (Bufo marinus) have been labelled with amiloride. The number of binding sites remained constant in suspensions for up to 100 hr. 2. In the presence of a protein synthesis inhibitor (cycloheximide, 0-5 mug/ml.) there was a decline in the density of binding sites was approximately exponential. Regression analysis gave a half-life of approximately 60 hr. 3. Aldosterone (5 X 10(-8) M) caused a significant (P less than 0-001) increase (50%) in the density of amiloride binding sites. Cells which had been treated with aldosterone had populations of binding sites which declined, in the presence of cycloheximide, at rates indistinguishable from those of untreated cells.

从蟾蜍（海蟾蜍）膀胱制备的分离上皮细胞膜中的钠进入位点已用氨氯吡脒进行标记。在悬浮液中，结合位点的数量在长达100小时内保持恒定。2. 在存在蛋白质合成抑制剂（环己酰亚胺，0 - 5微克/毫升）的情况下，结合位点的密度下降，大致呈指数关系。回归分析得出半衰期约为60小时。3. 醛固酮（5×10⁻⁸ M）使氨氯吡脒结合位点的密度显著增加（P＜0.001）（增加50%）。用醛固酮处理过的细胞，其结合位点群体在环己酰亚胺存在的情况下，下降速率与未处理细胞的速率无明显差异。