蛙皮上皮细胞中钠通道数量的上限。
An upper limit to the number of sodium channels in frog skin epithelium.
作者信息
Cuthbert A W
出版信息
J Physiol. 1973 Feb;228(3):681-92. doi: 10.1113/jphysiol.1973.sp010106.
Abstract
- Binding of [(14)C]amiloride to the mucosal surface of frog skin epithelium has been measured in the presence and absence of an excess of unlabelled amiloride. Simultaneous records were obtained of the sodium transport through the skin as indicated by the short circuit current.2. The number of binding sites with affinities for amiloride of 5 x 10(7) l./mole was around 400/mum(2).3. The relation between the binding sites and sodium channels is discussed.4. Making certain assumptions it is concluded that when frog skin epithelium is bathed in Ringer solution each sodium channel can handle 8000 sodium ions/sec.
摘要
在有无过量未标记氨氯地平存在的情况下，测定了[(14)C]氨氯地平与蛙皮上皮黏膜表面的结合情况。同时记录了通过短路电流表示的经皮肤的钠转运情况。
对氨氯地平亲和力为5×10(7)升/摩尔的结合位点数量约为400/μm(2)。
讨论了结合位点与钠通道之间的关系。
在做出某些假设的情况下得出结论，当蛙皮上皮浸泡在林格氏液中时，每个钠通道每秒可处理8000个钠离子。
相似文献
1
An upper limit to the number of sodium channels in frog skin epithelium.蛙皮上皮细胞中钠通道数量的上限。
J Physiol. 1973 Feb;228(3):681-92. doi: 10.1113/jphysiol.1973.sp010106.
2
Estimation of the density of sodium entry sites in frog skin epithelium from the uptake of [3H]benzamil.根据[3H]苯甲咪的摄取量估算蛙皮上皮中钠进入位点的密度。
J Physiol. 1979 Oct;295:477-90. doi: 10.1113/jphysiol.1979.sp012981.
3
Interdependence of the two borders in a sodium transporting epithelium. Possible regulation by the transport pool.钠转运上皮细胞中两个边界的相互依赖性。转运池的可能调节作用。
J Membr Biol. 1978;40 Spec No:221-45. doi: 10.1007/BF02026008.
4
Induction of transporting sites in a sodium transporting epithelium.在钠转运上皮细胞中诱导转运位点。
J Physiol. 1976 Aug;260(1):223-35. doi: 10.1113/jphysiol.1976.sp011512.
5
Amiloride as a probe for sodium entry sites in frog skin epithelium [proceedings].氨氯吡咪作为青蛙皮肤上皮钠进入位点的探针[会议论文集]
J Physiol. 1977 Mar;266(1):28P-29P.
6
Characteristics of the entry process for sodium in transporting epithelia as revealed with amiloride.用氨氯吡咪揭示的转运上皮细胞中钠进入过程的特征。
J Physiol. 1976 Mar;255(3):587-604. doi: 10.1113/jphysiol.1976.sp011297.
7
Uptake of [3H]benzamil at different sodium concentrations. Inferences regarding the regulation of sodium permeability.在不同钠浓度下[3H]苯扎明的摄取。关于钠通透性调节的推论。
J Physiol. 1979 Oct;295:491-504. doi: 10.1113/jphysiol.1979.sp012982.
8
Sodium uptake by frog skin and its modification by inhibitors of transepithelial sodium transport.蛙皮对钠的摄取及其经上皮钠转运抑制剂的调节作用。
J Physiol. 1973 Jan;228(1):221-39. doi: 10.1113/jphysiol.1973.sp010083.
9
Role of basolateral membrane conductance in the regulation of transepithelial sodium transport across frog skin.基底外侧膜电导在蛙皮跨上皮钠转运调节中的作用。
Pflugers Arch. 2003 May;446(2):198-202. doi: 10.1007/s00424-003-1009-z. Epub 2003 Jan 28.
10
Effect of amiloride on chloride transport across amphibian epithelia.氨氯吡咪对氯离子跨两栖类上皮细胞转运的影响。
J Membr Biol. 1978;40 Spec No:167-85. doi: 10.1007/BF02026004.
引用本文的文献
1
Irreversible inhibition of sodium transport by the toad urinary bladder following photolysis of amiloride analogs.氨氯地平类似物光解后蟾蜍膀胱对钠转运的不可逆抑制作用。
Experientia. 1981 Jan 15;37(1):68-9. doi: 10.1007/BF01965574.
2
Kinetics of amiloride action in the hen coprodaeum in vitro.氨氯地平在体外鸡泄殖腔中的作用动力学
Pflugers Arch. 1982 Feb;392(4):340-6. doi: 10.1007/BF00581629.
3
Control of sodium permeability of the outer barrier in toad skin.蟾蜍皮肤外层屏障钠通透性的控制
J Membr Biol. 1982;66(2):97-107. doi: 10.1007/BF01868486.
4
Amiloride inhibits mammalian renal kallikrein and a kallikrein-like enzyme from toad bladder and skin.氨氯吡咪抑制哺乳动物肾激肽释放酶以及蟾蜍膀胱和皮肤中的一种类激肽释放酶。
J Clin Invest. 1980 Jun;65(6):1343-50. doi: 10.1172/JCI109798.
5
Decreased sensitivity to amiloride of amphibian epithelia treated with aldosterone. Further evidence for an apical hormonal effect.用醛固酮处理的两栖动物上皮对氨氯吡脒的敏感性降低。关于顶端激素效应的进一步证据。
Pflugers Arch. 1980 Jan;383(2):151-8. doi: 10.1007/BF00581876.
6
The intracellular localization of amiloride in frog skin.阿米洛利在蛙皮中的细胞内定位。
Histochem J. 1983 Mar;15(3):239-55. doi: 10.1007/BF01006239.
7
Molecular actions of diuretics.利尿剂的分子作用
Klin Wochenschr. 1982 Oct 1;60(19):1258-63. doi: 10.1007/BF01716734.
8
Irreversible inhibition of epithelial sodium channels by ultraviolet irradiation.紫外线照射对上皮钠通道的不可逆抑制作用。
Br J Pharmacol. 1982 Nov;77(3):431-42. doi: 10.1111/j.1476-5381.1982.tb09315.x.
9
Amiloride-sensitive sodium channels in rabbit and guinea-pig gall-bladder.兔和豚鼠胆囊中的氨氯地平敏感钠通道。
J Physiol. 1982 May;326:21-34. doi: 10.1113/jphysiol.1982.sp014174.
10
Fluctuation analysis of short-circuit current in a warm-blooded sodium-retaining epithelium: site current, density, and interaction with triamterene.温血保钠上皮细胞短路电流的波动分析：部位电流、密度以及与氨苯蝶啶的相互作用
J Membr Biol. 1982;65(1-2):19-30. doi: 10.1007/BF01870465.
本文引用的文献
1
Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.钠的主动转运作为短路离体蛙皮电流的来源。
Acta Physiol Scand. 1951 Aug 25;23(2-3):110-27. doi: 10.1111/j.1748-1716.1951.tb00800.x.
2
Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.钠在离体蟾蜍膀胱黏膜表面的转运及其受血管加压素的影响。
J Gen Physiol. 1962 Jan;45(3):529-43. doi: 10.1085/jgp.45.3.529.
3
Transport and distribution of sodium across frog skin.钠在蛙皮中的转运与分布
J Physiol. 1967 Jun;190(3):481-97. doi: 10.1113/jphysiol.1967.sp008223.
4
An upper limit to the number of sodium channels in nerve membrane?神经膜中钠通道数量的上限？
J Physiol. 1967 Jan;188(1):99-105. doi: 10.1113/jphysiol.1967.sp008126.
5
The binding of tetrodotoxin to nerve membranes.河豚毒素与神经膜的结合。
J Physiol. 1971 Feb;213(1):235-54. doi: 10.1113/jphysiol.1971.sp009379.
6
The low-frequency electrical impedance of the isolated frog skin.离体蛙皮的低频电阻抗
Acta Physiol Scand. 1971 Mar;81(3):355-66. doi: 10.1111/j.1748-1716.1971.tb04910.x.
7
Effect of amiloride on sodium transport in frog skin. II. Sodium transport pool and unidirectional fluxes.氨氯吡咪对蛙皮钠转运的影响。II. 钠转运池与单向通量
Pflugers Arch. 1970;321(2):91-101. doi: 10.1007/BF00586365.
8
The effects of saponins on the transporting epithelium of isolated frog skin.皂苷对离体蛙皮转运上皮的作用。
Br J Pharmacol. 1970 Nov;40(3):544P.
9
Effect of Amiloride on sodium transport of frog skin. I. Action on intracellular sodium content.氨氯吡咪对蛙皮钠转运的作用。I. 对细胞内钠含量的作用
Pflugers Arch. 1970;317(1):84-92. doi: 10.1007/BF00586701.
10
Changes in sodium pool and kinetics of sodium transport in frog skin produced by amiloride.氨氯吡咪对蛙皮钠池及钠转运动力学的影响。
Br J Pharmacol. 1970 May;39(1):99-109. doi: 10.1111/j.1476-5381.1970.tb09559.x.
Zotero 插件