Zhang P, Spradling A C
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, MD 21210.
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3539-43. doi: 10.1073/pnas.91.9.3539.
Insertional mutagenesis with transposable P elements has greatly facilitated the identification and analysis of genes located throughout the 70% of the Drosophila melanogaster genome classified as euchromatin. In contrast, genetically marked P elements have only rarely been shown to transpose into heterochromatin. By carrying out single P element insertional mutagenesis under conditions where position-effect variegation was suppressed, we efficiently generated strains containing insertions at diverse sites within centromeric and Y-chromosome heterochromatin. The tendency of P elements to transpose locally was shown to operate within heterochromatin, and it further enhanced the recovery of heterochromatic insertions. Three of the insertions disrupted vital genes known to be present at low density in heterochromatin. Strains containing single P element insertions will greatly facilitate the structural and functional analysis of this poorly understood genomic component.
利用可转座的P因子进行插入诱变，极大地促进了对位于果蝇黑腹果蝇基因组70%被归类为常染色质区域内基因的鉴定和分析。相比之下，带有遗传标记的P因子很少能转座到异染色质中。通过在位置效应斑驳被抑制的条件下进行单个P因子插入诱变，我们有效地产生了在着丝粒和Y染色体异染色质内不同位点含有插入的菌株。P因子在局部转座的倾向在异染色质内也起作用，并且进一步提高了异染色质插入的回收率。其中三个插入破坏了已知在异染色质中低密度存在的重要基因。含有单个P因子插入的菌株将极大地促进对这个了解甚少的基因组组分的结构和功能分析。
