University of Melbourne, Parkville 3010, Melbourne, Australia.
Cells. 2012 Dec 5;1(4):1182-96. doi: 10.3390/cells1041182.
Animal growth requires coordination of cell growth and cell cycle progression with developmental signaling. Loss of cell cycle control is extremely detrimental, with reduced cycles leading to impaired organ growth and excessive proliferation, potentially resulting in tissue overgrowth and driving tumour initiation. Due to the high level of conservation between the cell cycle machinery of Drosophila and humans, the appeal of the fly model continues to be the means with which we can use sophisticated genetics to provide novel insights into mammalian growth and cell cycle control. Over the last decade, there have been major additions to the genetic toolbox to study development in Drosophila. Here we discuss some of the approaches available to investigate the potent growth and cell cycle properties of the Drosophila counterparts of prominent cancer genes, with a focus on the c-Myc oncoprotein and the tumour suppressor protein FIR (Hfp in flies), which behaves as a transcriptional repressor of c-Myc.
动物的生长需要协调细胞生长和细胞周期进程与发育信号。失去细胞周期控制是极其有害的，减少周期会导致器官生长受损和过度增殖，可能导致组织过度生长并驱动肿瘤起始。由于果蝇和人类的细胞周期机制之间存在高度的保守性，因此蝇模型的吸引力仍然是我们可以利用复杂的遗传学提供对哺乳动物生长和细胞周期控制的新见解的手段。在过去的十年中，已经有了更多的遗传工具来研究果蝇的发育。在这里，我们讨论了一些可用于研究重要癌症基因的果蝇对应物的强大生长和细胞周期特性的方法，重点介绍了 c-Myc 癌蛋白和肿瘤抑制蛋白 FIR（果蝇中的 Hfp），它作为 c-Myc 的转录抑制剂。
