De Togni P, Goellner J, Ruddle N H, Streeter P R, Fick A, Mariathasan S, Smith S C, Carlson R, Shornick L P, Strauss-Schoenberger J
Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Science. 1994 Apr 29;264(5159):703-7. doi: 10.1126/science.8171322.
Mice rendered deficient in lymphotoxin (LT) by gene targeting in embryonic stem cells have no morphologically detectable lymph nodes or Peyer's patches, although development of the thymus appears normal. Within the white pulp of the spleen, there is failure of normal segregation of B and T cells. Spleen and peripheral blood contain CD4+CD8- and CD4-CD8+ T cells in a normal ratio, and both T cells subsets have an apparently normal lytic function. Lymphocytes positive for immunoglobulin M are present in increased numbers in both the spleen and peripheral blood. These data suggest an essential role for LT in the normal development of peripheral lymphoid organs.
通过对胚胎干细胞进行基因靶向操作，使淋巴毒素（LT）缺乏的小鼠没有形态上可检测到的淋巴结或派尔集合淋巴结，尽管胸腺发育看起来正常。在脾脏白髓内，B细胞和T细胞无法正常分离。脾脏和外周血中CD4⁺CD8⁻和CD4⁻CD8⁺ T细胞的比例正常，且两个T细胞亚群的裂解功能明显正常。脾脏和外周血中免疫球蛋白M阳性的淋巴细胞数量均增加。这些数据表明LT在外周淋巴器官的正常发育中起重要作用。
