Crestani B, Seta N, De Bandt M, Soler P, Rolland C, Dehoux M, Boutten A, Dombret M C, Palazzo E, Kahn M F
Unité INSERM 226, Hôpital Bichat, Paris, France.
Am J Respir Crit Care Med. 1994 May;149(5):1260-5. doi: 10.1164/ajrccm.149.5.8173768.
Interleukin-6 (IL-6) has been involved in the pathogenesis of inflammatory and/or autoimmune diseases. We characterized the production of IL-6 by blood monocytes and alveolar macrophages (AM) in 11 patients with definite systemic sclerosis (SSc) with lung involvement and in eight normal control subjects. IL-6 levels were determined in serum, bronchoalveolar lavage (BAL) supernatants, monocytes, and AM cell culture supernatants using an ELISA kit. BAL cell analysis evidenced an alveolitis with hypercellularity and increased neutrophils and mast cells absolute counts. Serum and BAL IL-6 levels were low and similar in SSc and control groups. The monocytes of the group with SSc secreted more IL-6 than did the control group, both spontaneously (p = 0.01) and after LPS stimulation (p = 0.0007). Spontaneous secretion of IL-6 by AM tended to be higher in the SSc group than in the control group (p = 0.22). LPS-induced IL-6 secretion by AM was similar in both groups. Our study demonstrates that during SSc lung disease, spontaneous and stimulated IL-6 secretion by blood monocytes is increased, compared with secretion by healthy control subjects. By contrast, IL-6 secretion by AM is normal despite evidence of mild alveolitis.
白细胞介素-6（IL-6）参与了炎症性和/或自身免疫性疾病的发病机制。我们对11例确诊为合并肺部受累的系统性硬化症（SSc）患者及8名正常对照者的血液单核细胞和肺泡巨噬细胞（AM）产生IL-6的情况进行了特征分析。使用酶联免疫吸附测定（ELISA）试剂盒测定血清、支气管肺泡灌洗（BAL）上清液、单核细胞及AM细胞培养上清液中的IL-6水平。BAL细胞分析显示存在肺泡炎，细胞增多，中性粒细胞和肥大细胞绝对计数增加。SSc组和对照组的血清及BAL中IL-6水平较低且相似。SSc组单核细胞自发分泌的IL-6比对照组更多（p = 0.01），脂多糖（LPS）刺激后也是如此（p = 0.0007）。SSc组AM自发分泌IL-6的水平倾向于高于对照组（p = 0.22）。两组中LPS诱导的AM分泌IL-6水平相似。我们的研究表明，在SSc肺部疾病期间，与健康对照者相比，血液单核细胞自发和受刺激分泌IL-6均增加。相比之下，尽管有轻度肺泡炎的证据，但AM分泌IL-6正常。
