Choudhury N, Wall P M, Truswell A S
Department of Biochemistry, University of Sydney, New South Wales, Australia.
Clin Chem. 1994 May;40(5):710-5.
A single blood cholesterol measurement may not accurately reflect an individual's true mean concentration. If duplicate blood samples are taken, what number of days between sampling gives the best chance of detecting the maximum within-subject variation? In this study, we analyzed 20 serial blood samples obtained from each of 13 healthy, menstruating women over 35 days. Variability was calculated as the semivariogram, which gives the average squared difference between replicate samples taken over a range of sampling intervals. Data were available for a complete set of intervals from 1 to 26 days. Variability in total cholesterol (TC) increased as the interval between sampling increased from 1 to 12 days. With high-density lipoprotein cholesterol (HDL-C), variability increased from 1- to 7-day intervals. In practice, our results suggest that, irrespective of the time of menstruation, the minimal interval for collecting a second blood sample for TC and HDL-C assays is approximately 2 weeks.
单次血液胆固醇测量可能无法准确反映个体的真实平均浓度。如果采集两份血液样本，采样间隔几天能最有效地检测出个体内的最大差异？在本研究中，我们分析了13名35岁以上健康育龄女性在35天内每人采集的20份连续血液样本。变异性通过半变异函数计算得出，它给出了在一系列采样间隔内重复样本之间的平均平方差。有完整的1至26天采样间隔的数据。总胆固醇（TC）的变异性随着采样间隔从1天增加到12天而增加。对于高密度脂蛋白胆固醇（HDL-C），变异性在1至7天的间隔内增加。在实际操作中，我们的结果表明，无论月经时间如何，采集第二份血液样本进行TC和HDL-C检测的最短间隔约为2周。
