通过脑脊液DNA扩增诊断爱泼斯坦-巴尔病毒引起的中枢神经系统感染。

Diagnosis of Epstein-Barr virus-induced central nervous system infections by DNA amplification from cerebrospinal fluid.

作者信息

Landgren M, Kyllerman M, Bergström T, Dotevall L, Ljungström L, Ricksten A

机构信息

Department of Pediatrics II, University of Göteborg, Sweden.

出版信息

Ann Neurol. 1994 May;35(5):631-5. doi: 10.1002/ana.410350522.

DOI:10.1002/ana.410350522

PMID:8179310

Abstract

A nested polymerase chain reaction was used for the detection of Epstein-Barr virus DNA in 1 patient with encephalitis, and in 1 patient with myelitis. Epstein-Barr virus DNA was detected in cerebrospinal fluid samples obtained at the onset of neurological symptoms in both patients, and serological findings indicated ongoing Epstein-Barr virus infection. In the patient with encephalitis, herpes simplex virus type 1 DNA was transiently detected in the cerebrospinal fluid, while Epstein-Barr virus DNA was still present on day 44 after admittance. Single-photon emission computed tomography in this patient indicated a frontal bilateral hypoperfusion. The diagnostic value of polymerase chain reaction on cerebrospinal fluid and serum samples for Epstein-Barr virus infections of the central nervous system is emphasized.

摘要

采用巢式聚合酶链反应检测1例脑炎患者和1例脊髓炎患者的EB病毒DNA。在两名患者出现神经症状时采集的脑脊液样本中均检测到EB病毒DNA，血清学检查结果表明存在持续性EB病毒感染。在脑炎患者中，脑脊液中短暂检测到1型单纯疱疹病毒DNA，而入院44天后仍存在EB病毒DNA。该患者的单光子发射计算机断层扫描显示双侧额叶灌注不足。强调了聚合酶链反应对脑脊液和血清样本在中枢神经系统EB病毒感染诊断中的价值。