Armstrong A S, Mathias J R, DeYoung M I, Hirata A A
Infect Immun. 1979 Apr;24(1):51-8. doi: 10.1128/iai.24.1.51-58.1979.
A reverse passive hemagglutination test that utilizes human erythrocytes coated with antibody to gonococci was developed to distinguish differences among 11 strains of Neisseria gonorrhoeae. Different rabbits were immunized with each strain of gonococcus. Antibody was purified by passing antiserum over an immunoadsorbent column containing homologous cell walls trapped in a cross-linked polyacrylamide gel. Antibody, after absorption with N. meningitidis, was used for coating 11 individual suspensions of erythrocytes, each with antibody to one gonococcal strain. The panel of coated erythrocytes was added to microtiter trays containing dilutions of homologous bacterial lysate and lysates from 10 heterologous strains. Agglutination titers were highest with homologous lysates, although cross-reactions occurred among some heterologous lysates. Lysates of nongonococcal Neisseria species and of other genera did not agglutinate coated erythrocytes. The reverse passive hemagglutination test can be a useful procedure to distinguish differences among strains of N. gonorrhoeae.
开发了一种反向被动血凝试验，该试验利用涂有抗淋球菌抗体的人红细胞来区分11株淋病奈瑟菌之间的差异。用每株淋球菌分别免疫不同的兔子。通过使抗血清通过含有捕获在交联聚丙烯酰胺凝胶中的同源细胞壁的免疫吸附柱来纯化抗体。用脑膜炎奈瑟菌吸收后的抗体用于包被11个单独的红细胞悬液，每个悬液含有针对一种淋球菌菌株的抗体。将包被的红细胞板加入到含有同源细菌裂解物和10种异源菌株裂解物稀释液的微量滴定板中。同源裂解物的凝集效价最高，尽管在一些异源裂解物之间发生了交叉反应。非淋球菌奈瑟菌属和其他属的裂解物不会使包被的红细胞凝集。反向被动血凝试验可能是区分淋病奈瑟菌菌株差异的有用方法。
