Buchanan T M
J Exp Med. 1975 Jun 1;141(6):1470-5. doi: 10.1084/jem.141.6.1470.
Pili were isolated from two different strains of gonococci (33 and 2686) and demonstrated to be pure by the criteria of electron microscopy and polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Each purified pili preparation was studied for its ability to inhibit; (a) binding of 124-I-labeled purified 2686 pili by antibody to 2686 pili, and (b) binding of 125-I-labeled purified 33 pili by antibody to 33 pili. In each instance progressive inhibition of binding was produced by homologous pili type, but no significant inhibition was observed using comparable weights of the heterologous pili type. These results indicate that the pili of strains 2686 and 33 are antigenically different.
从两种不同的淋病菌株（33株和2686株）中分离出菌毛，并通过电子显微镜和十二烷基硫酸钠聚丙烯酰胺凝胶电泳标准证明其为纯品。对每种纯化的菌毛制剂进行了如下抑制能力研究：（a）用抗2686菌毛的抗体抑制124-I标记的纯化2686菌毛的结合，以及（b）用抗33菌毛的抗体抑制125-I标记的纯化33菌毛的结合。在每种情况下，同源菌毛类型都能产生逐渐的结合抑制，但使用相同重量的异源菌毛类型未观察到明显抑制。这些结果表明，2686株和33株的菌毛在抗原性上是不同的。
