Rzepecki L M, Waite J H
College of Marine Studies, University of Delaware, Lewes 19958.
Mol Mar Biol Biotechnol. 1993 Oct;2(5):267-79.
The byssus of the zebra mussel Dreissena polymorpha is the key element of its adhesive strategy. It consists of a bundle of threads tipped by adhesive plaques and attached to the mussel at the base of its byssal-synthesizing organ, the foot. Two polyphenolic protein precursors of the byssus have been purified from the foot. These precursors, Dpfp-1 and Dpfp-2 (Dreissena polymorpha foot protein), with apparent M(r) values of 76 and 26 K, respectively, contain 3,4-dihydroxyphenylalanine (DOPA) integrated into their primary sequence, but differ from previously characterized polyphenolic proteins from marine mussels. The related quagga mussel (Dreissena spp.?) has homologous proteins with significantly different compositions. The zebra mussel DOPA proteins are tandemly repetitive with unique oligopeptide motif sequences, contain tryptophan, and are O-glycosylated primarily on threonine residues. Galactosamine was the only carbohydrate detected after hydrolysis. Dpfp-1 constitutes a polymorphic family of polypeptides with, unusually, an acidic range of pI values between 5.3 and 6.5. The detection of carbohydrate in the thread and in the juncture between thread and plaque suggests that these two proteins are localized in those regions where they may function as lacquers or structural elements.
斑马贻贝（Dreissena polymorpha）的足丝是其附着策略的关键要素。它由一束带有粘性斑块的细丝组成，这些细丝在足丝合成器官（足部）的基部与贻贝相连。已经从足部纯化出了两种足丝的多酚蛋白前体。这些前体，即Dpfp - 1和Dpfp - 2（斑马贻贝足部蛋白），其表观分子量（M(r)）值分别为76k和26k，在其一级序列中含有3,4 - 二羟基苯丙氨酸（多巴，DOPA），但与先前表征的海洋贻贝的多酚蛋白不同。相关的斑马贝（Dreissena spp.?）具有组成明显不同的同源蛋白。斑马贻贝的多巴蛋白具有独特的寡肽基序序列串联重复，含有色氨酸，并且主要在苏氨酸残基上进行O - 糖基化。水解后检测到的唯一碳水化合物是半乳糖胺。Dpfp - 1构成了一个多态性的多肽家族，其pI值异常地在5.3至6.5的酸性范围内。在细丝以及细丝与斑块之间的连接处检测到碳水化合物，这表明这两种蛋白定位于它们可能作为漆或结构元件发挥作用的区域。
