Russell J R, Hosein F, Johnson E, Waugh R, Powell W
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Mol Ecol. 1993 Apr;2(2):89-97. doi: 10.1111/j.1365-294x.1993.tb00003.x.
In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini-prep DNA extraction procedure together with a polymerase-chain-reaction- (PCR)-based polymorphic assay procedure (RAPD). Twenty-five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed.
为了保护和利用热带森林树木（如可可）的宝贵遗传资源，有必要对现有的遗传变异性进行系统评估。我们所采用的方法基于一种简单的微量制备DNA提取程序以及一种基于聚合酶链反应（PCR）的多态性检测程序（随机扩增多态性DNA，RAPD）。从秘鲁收集的25份可可种质（IMCs和PAs）以及从厄瓜多尔收集的LCTEENs，利用形态学或生化描述符难以区分，使用最少三种寡核苷酸引物对其进行了独特的指纹识别。对使用RAPD检测到的变异性进行分析，清楚地区分了这三个可可种群的地理起源。将变异性划分为种群内和种群间成分，结果表明大部分变异存在于种群内。讨论了RAPD分析在促进田间基因库合理化以及提供准确的多样性估计以优化收集策略方面的潜力。
