模仿葡萄球菌溶血型生物变种的质粒编码过氧化氢酶

Plasmid-encoded catalase of Staphylococcus simulans biovar staphylolyticus.

作者信息

Fondren L M, Sloan G L, LeBlanc P A, Heath H E

机构信息

Department of Biological Sciences, University of Alabama, Tuscaloosa 35487.

出版信息

FEMS Microbiol Lett. 1994 Apr 1;117(2):231-5. doi: 10.1016/0378-1097(94)90200-3.

DOI:10.1016/0378-1097(94)90200-3

PMID:8181729

Abstract

Staphylococcus simulans biovar staphylolyticus contains five plasmids designated pACK1 through pACK5. Non-denaturing electrophoretic analysis of an extract prepared from wild-type cells revealed three bands of catalase activity, whereas an extract of cells cured of pACK1 produced only two catalase bands. Cloning and Southern hybridization analysis showed that there is a catalase structural gene on pACK1. The plasmid-specified catalase was the major activity produced under both aerobic and anaerobic conditions of growth.

摘要

模仿葡萄球菌溶葡萄球菌生物变种含有五个被命名为pACK1至pACK5的质粒。对野生型细胞提取物进行的非变性电泳分析显示出三条过氧化氢酶活性带，而缺失pACK1的细胞提取物仅产生两条过氧化氢酶带。克隆和Southern杂交分析表明，pACK1上存在一个过氧化氢酶结构基因。质粒指定的过氧化氢酶是在有氧和无氧生长条件下产生的主要活性物质。