白色念珠菌t-RNA连接酶编码基因的分离与测序。

Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans.

作者信息

Baymiller J, Jennings S, Kienzle B, Gorman J A, Kelly R, McCullough J E

机构信息

Department of Microbial Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.

出版信息

Gene. 1994 May 3;142(1):129-34. doi: 10.1016/0378-1119(94)90367-0.

DOI:10.1016/0378-1119(94)90367-0

PMID:8181747

Abstract

The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.

摘要

通过对含有编码tRNA连接酶的结构基因RLG1被破坏的酿酒酵母菌株进行互补，从白色念珠菌的基因组文库中分离出编码tRNA连接酶的基因。克隆的基因也能互补RLG1的温度敏感等位基因。序列分析揭示了一个单一的2499个核苷酸的编码区。该基因编码一个由833个氨基酸组成的蛋白质，与酿酒酵母tRNA连接酶有42%的同源性。通过脉冲场凝胶电泳分离的白色念珠菌染色体杂交将该基因定位到1号染色体，即白色念珠菌最小的染色体上。