Boekhoff I, Michel W C, Breer H, Ache B W
Institute of Zoophysiology, University of Stuttgart-Hohenheim, Germany.
J Neurosci. 1994 May;14(5 Pt 2):3304-9. doi: 10.1523/JNEUROSCI.14-05-03304.1994.
Quench-flow measurements are used to determine the subsecond kinetics of odor-induced changes in second messenger concentrations in lobster olfactory receptor neurons. Individual odors transiently and differentially increase the production of both adenosine cAMP and inositol 1,4,5-trisphosphate (IP3) within 50 msec of odor stimulation. The ability of two different odors to stimulate cAMP and IP3 correlates with the odors' ability to excite and inhibit receptor cells physiologically. These results strengthen the proposition, heretofore based largely on evidence from cultured cells, that dual second messenger pathways mediate excitatory and inhibitory input to lobster olfactory receptor cells.
淬灭流测量法用于确定龙虾嗅觉受体神经元中气味诱导的第二信使浓度变化的亚秒级动力学。在气味刺激的50毫秒内，单一气味会瞬时且不同程度地增加腺苷环磷酸（cAMP）和肌醇-1,4,5-三磷酸（IP3）的生成。两种不同气味刺激cAMP和IP3的能力与这些气味在生理上兴奋和抑制受体细胞的能力相关。这些结果强化了此前主要基于培养细胞证据得出的观点，即双重第二信使途径介导了对龙虾嗅觉受体细胞的兴奋性和抑制性输入。
