Kotsuji F, Kubo M, Tominaga T
Department of Obstetrics and Gynecology, Fukui Medical School Matsuoka-Cho, Japan.
J Reprod Fertil. 1994 Jan;100(1):151-6. doi: 10.1530/jrf.0.1000151.
An in vitro culture system in which two types of cell from ovarian follicular walls were attached to opposite surfaces of a collagen membrane was developed. The effect of granulosa-theca cell communication on oocyte maturation was investigated using this culture system. Bovine cumulus-oocyte complexes were incubated with granulosa cells, with or without theca cells. The time course of germinal vesicle breakdown was assessed. When cultured with granulosa cells alone, oocytes showed 100% germinal vesicle breakdown after incubation for 6 h. However, when cultured with granulosa and theca cells, germinal vesicle breakdown was significantly delayed for up to 12 h. Breakdown was further delayed by 12 h when cumulus-oocyte complexes were incubated with FSH-treated granulosa and thecal cultures. FSH did not exert this inhibitory effect when in culture with granulosa cells alone. When cumulus-oocyte complexes were incubated with thecal cells alone, all oocytes underwent germinal vesicle breakdown within 6 h; the addition of FSH to the culture medium did not affect the time course of breakdown. It is concluded that a signal(s) from thecal cells augments the meiosis-arresting activity of granulosa cells and that FSH increases the meiosis-arresting activity of granulosa cells under the influence of thecal cells.
开发了一种体外培养系统，其中来自卵巢卵泡壁的两种类型的细胞附着在胶原膜的相对表面上。使用该培养系统研究了颗粒-卵泡膜细胞通讯对卵母细胞成熟的影响。将牛卵丘-卵母细胞复合体与颗粒细胞一起培养，有或没有卵泡膜细胞。评估生发泡破裂的时间进程。当单独与颗粒细胞一起培养时，卵母细胞在孵育6小时后显示100%的生发泡破裂。然而，当与颗粒细胞和卵泡膜细胞一起培养时，生发泡破裂显著延迟长达12小时。当卵丘-卵母细胞复合体与经促卵泡素（FSH）处理的颗粒细胞和卵泡膜细胞培养物一起孵育时，破裂进一步延迟12小时。当单独与颗粒细胞培养时，FSH不会发挥这种抑制作用。当卵丘-卵母细胞复合体仅与卵泡膜细胞一起孵育时，所有卵母细胞在6小时内都经历了生发泡破裂；向培养基中添加FSH不会影响破裂的时间进程。得出的结论是，来自卵泡膜细胞的一种或多种信号增强了颗粒细胞的减数分裂阻滞活性，并且FSH在卵泡膜细胞的影响下增加了颗粒细胞的减数分裂阻滞活性。
