Van Lambalgen A A, van Kraats A A, Mulder M F, Teerlink T, van den Bos G C
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
Am J Physiol. 1994 Apr;266(4 Pt 2):H1581-7. doi: 10.1152/ajpheart.1994.266.4.H1581.
Endotoxemia can affect the storage of high-energy phosphates [ATP, creatine phosphate (CrP)] even in organs in which global blood flow does not fall. If a decrease in this storage is due to an inadequate oxygen supply-to-demand ratio, improving the perfusion should restore it. Therefore, in anesthetized endotoxemic rats we studied organ perfusion and the storage of high-energy phosphates of heart, liver, kidney, and skeletal muscle and measured the effects of improving cardiac output (CO) and organ blood flow with cardiostimulatory drugs [dopexamine (DX) and dobutamine (DB)]. Endotoxin (Escherichia coli O127.B8, 8 mg/kg) was infused from 0 to 60 min in three groups of anesthetized rats: one untreated (saline only) group (ES; n = 10), and two groups in which we infused DX (3 x 10(-8) mol.kg-1.min-1; n = 10) or DB (10(-7) mol.kg-1.min-1; n = 8) from 60 to 135 min. A fourth group served as time-matched controls (C; n = 8). Organ blood flows at 0 and 135 min (end of experiment) were measured with radioactive microspheres. In biopsies (at 135 min) we measured lactate, ATP, and CrP concentrations. Endotoxemia decreased CO (45% at 135 min; P < 0.05), which could be restored by DX and DB. Myocardial and skeletal muscle blood flow and ATP did not differ in the groups at 135 min. Hepatic and renal blood flow decreased in the ES group 44 and 52%, respectively (P < 0.05); DX restored the fall of hepatic and DB of renal blood flow.(ABSTRACT TRUNCATED AT 250 WORDS)
内毒素血症即使在整体血流量未下降的器官中也会影响高能磷酸盐（三磷酸腺苷、磷酸肌酸）的储存。如果这种储存量的减少是由于氧供需比不足所致，那么改善灌注应该可以使其恢复。因此，在麻醉的内毒素血症大鼠中，我们研究了心脏、肝脏、肾脏和骨骼肌的器官灌注及高能磷酸盐的储存情况，并测量了使用心脏兴奋药物（多培沙明和多巴酚丁胺）提高心输出量和器官血流量的效果。在三组麻醉大鼠中，于0至60分钟内输注内毒素（大肠杆菌O127.B8，8毫克/千克）：一组未治疗（仅输注生理盐水）组（ES组；n = 10），以及两组分别在60至135分钟内输注多培沙明（3×10⁻⁸摩尔·千克⁻¹·分钟⁻¹；n = 10）或多巴酚丁胺（10⁻⁷摩尔·千克⁻¹·分钟⁻¹；n = 8）的组。第四组作为时间匹配对照组（C组；n = 8）。在实验开始时（0分钟）和结束时（135分钟）用放射性微球测量器官血流量。在活检时（135分钟）测量乳酸、三磷酸腺苷和磷酸肌酸浓度。内毒素血症使心输出量下降（135分钟时下降45%；P < 0.05），多培沙明和多巴酚丁胺可使其恢复。在135分钟时，各组间心肌和骨骼肌血流量及三磷酸腺苷水平无差异。ES组肝脏和肾脏血流量分别下降44%和52%（P < 0.05）；多培沙明使肝脏血流量下降恢复，多巴酚丁胺使肾脏血流量下降恢复。（摘要截断于250字）
