Tanabe A, Naruse M, Arai K, Naruse K, Yoshimoto T, Seki T, Imaki T, Kobayashi M, Miyazaki H, Demura H
Department of Medicine, Tokyo Women's Medical University, Japan.
J Endocrinol Invest. 1998 Nov;21(10):668-72. doi: 10.1007/BF03350796.
Angiotensin II (Ang II) type 2 receptor (AT2) has been shown to counteract the type 1 receptor (AT1)-mediated biological actions of Ang II in the cardiovascular system. The biological significance of AT2 receptor in the adrenals however remains unknown. In the present study, we investigated the roles of AT1 and AT2 receptor subtypes in the regulation of aldosterone secretion and DNA synthesis in bovine adrenocortical zona glomerulosa cells in vitro. Ang II (1 mumol/l)-stimulated aldosterone secretion was completely suppressed by AT1 antagonist CV-11974 but not affected by AT2 receptor antagonist PD-123319. Effects on DNA synthesis were investigated by determining the incorporation of BrdU into the nuclei of the cultured zona glomerulosa cells. Ang II (1 mumol/l)-stimulated DNA synthesis of the cells was also completely suppressed by CV-11974 but not by PD-123319. These results suggest that AT1 receptor but not AT2 receptor is the predominant receptor subtype which mediates the Ang II-stimulated aldosterone secretion and cell growth in bovine adrenocortical cells.
血管紧张素II（Ang II）2型受体（AT2）已被证明可抵消1型受体（AT1）介导的Ang II在心血管系统中的生物学作用。然而，AT2受体在肾上腺中的生物学意义仍然未知。在本研究中，我们在体外研究了AT1和AT2受体亚型在调节牛肾上腺皮质球状带细胞醛固酮分泌和DNA合成中的作用。Ang II（1μmol/L）刺激的醛固酮分泌被AT1拮抗剂CV-11974完全抑制，但不受AT2受体拮抗剂PD-123319的影响。通过测定BrdU掺入培养的球状带细胞核中的情况来研究对DNA合成的影响。Ang II（1μmol/L）刺激的细胞DNA合成也被CV-11974完全抑制，但未被PD-123319抑制。这些结果表明，AT1受体而非AT2受体是介导Ang II刺激牛肾上腺皮质细胞醛固酮分泌和细胞生长的主要受体亚型。
