Eckloff B W, Podzorski R P, Kline B C, Cockerill F R
Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
Int J Syst Bacteriol. 1994 Apr;44(2):320-3. doi: 10.1099/00207713-44-2-320.
Other workers have found that clinical isolates of Helicobacter pylori exhibit very extensive DNA sequence polymorphisms when they are examined by ribotyping or some other genomic sequence characterization technique. In fact, it is rare to find similar clones, much less identical ones, among isolates. We found that the levels of divergence between the 16S ribosomal DNA sequences of individual organisms and the consensus sequence of the five isolates which we examined ranged from 0.2 to 0.5%. In contrast, other workers have shown that levels of divergence between the 16S ribosomal DNA sequence of H. pylori and the 16S ribosomal DNA sequences of four other Helicobacter species range from 2.7 to 8.0%. Our results show that the H. pylori 16S ribosomal DNA is not very polymorphic and support the conclusion that H. pylori is a unique species.
其他研究人员发现，当通过核糖体分型或其他一些基因组序列表征技术检测时，幽门螺杆菌的临床分离株表现出非常广泛的DNA序列多态性。事实上，在分离株中很少能找到相似的克隆，更不用说完全相同的克隆了。我们发现，个体生物的16S核糖体DNA序列与我们检测的五个分离株的共有序列之间的差异水平在0.2%至0.5%之间。相比之下，其他研究人员表明，幽门螺杆菌的16S核糖体DNA序列与其他四种螺杆菌属物种的16S核糖体DNA序列之间的差异水平在2.7%至8.0%之间。我们的结果表明，幽门螺杆菌的16S核糖体DNA不是非常多态，并支持幽门螺杆菌是一个独特物种的结论。
