Wilkinson S M, Uhl J R, Kline B C, Cockerill F R
Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA.
J Clin Pathol. 1998 Feb;51(2):127-33. doi: 10.1136/jcp.51.2.127.
Recent studies suggest that Helicobacter pylori is an invasive enteropathogen. However, the efficiency with which this pathogen invades mammalian cells remains unknown. Therefore, this study was designed to investigate the invasion frequencies of HEp-2 cells by clinical strains of H pylori.
An acridine orange assay and cultured HEp-2 cell monolayers were used to determine the HEp-2 cell penetration frequencies of 17 clinical isolates and one American Type Culture Collection (ATCC) strain of H pylori, and single clinical strains of Yersinia enterocolitica, Shigella flexneri, and a non-invasive ATCC Escherichia coli strain.
The acridine orange assay demonstrated that invasion frequencies of HEp-2 cells by all H pylori isolates were significant and, in most instances, exceeded those for the S flexneri strain and equalled those for the Y enterocolitica strain. The assay also showed that internalised H pylori organisms remained viable for at least six hours, the maximum time that bacteria and HEp-2 cells were co-incubated.
These results may have important implications for treatment and prevention strategies for this gastric pathogen. Furthermore, the acridine orange assay may be useful for assessing, in vitro, the ability of conventional and newer antibiotics, alone or in combination, to kill intracellular H pylori organisms.
近期研究表明幽门螺杆菌是一种侵袭性肠道病原体。然而，这种病原体侵袭哺乳动物细胞的效率仍不清楚。因此，本研究旨在调查幽门螺杆菌临床菌株对人喉表皮样癌细胞（HEp-2细胞）的侵袭频率。
采用吖啶橙检测法和培养的HEp-2细胞单层，来测定17株幽门螺杆菌临床分离株、1株美国典型培养物保藏中心（ATCC）菌株以及小肠结肠炎耶尔森菌、福氏志贺菌的单株临床菌株和1株非侵袭性ATCC大肠杆菌菌株对HEp-2细胞的穿透频率。
吖啶橙检测法表明，所有幽门螺杆菌分离株对HEp-2细胞的侵袭频率均显著，且在大多数情况下，超过了福氏志贺菌菌株的侵袭频率，与小肠结肠炎耶尔森菌菌株的侵袭频率相当。该检测还表明，内化的幽门螺杆菌至少在6小时内保持存活，这是细菌与HEp-2细胞共同孵育的最长时间。
这些结果可能对这种胃部病原体的治疗和预防策略具有重要意义。此外，吖啶橙检测法可能有助于在体外评估常规抗生素和新型抗生素单独或联合使用时杀死细胞内幽门螺杆菌的能力。
