Buczolits Sandra, Hirt Reinhard, Rosengarten Renate, Busse Hans-Jürgen
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210.Vienna, Austria
Vet Microbiol. 2003 Sep 24;95(4):259-70. doi: 10.1016/s0378-1135(03)00182-2.
The canine gastric mucosa is known to be a habitat for various Helicobacter species. So far, five Helicobacter species have been described from the canine gastric mucosa, but histological studies have demonstrated a greater variety. In order to gain more information on diversity of canine gastric mucosa colonising helicobacters, biopsy samples of four pet dogs were examined by DNA-based techniques. PCR with a primer pair binding specifically to the 16S rDNA of the species of the genus Helicobacter and generating a fragment of approximately 400 bp indicated the presence of Helicobacter strains in the stomachs of the four dogs. PCR products were cloned into Escherichia coli DH10B and PCR-re-amplified 16S rDNA fragments were subjected to amplified ribosomal DNA restriction analysis (ARDRA) employing restriction enzyme HhaI. Restriction profiles indicated the presence of at least two different Helicobacter species in two dogs. Partial sequences of 16S rDNA of six clones were compared with sequences available in the EMBL data bank. Two sequences obtained from different dogs were identical with the corresponding sequences of Helicobacter pylori strains. Three sequences showed highest but moderate similarity values to H. pylori (96.6-98.0%) and one sequence to Helicobacter salomonis (97.3%). In contrast to previous reports our data implicate that the gastric mucosa of dogs may be colonised by strains of H. pylori or a very closely related species but they also confirm indications for the presence of so far uncultivated species of Helicobacter.
犬胃黏膜是多种幽门螺杆菌的栖息地。到目前为止，已从犬胃黏膜中鉴定出五种幽门螺杆菌，但组织学研究表明其种类更多。为了获取更多关于犬胃黏膜定植幽门螺杆菌多样性的信息，采用基于DNA的技术对四只宠物犬的活检样本进行了检测。用一对特异性结合幽门螺杆菌属物种16S rDNA并产生约400 bp片段的引物进行PCR，结果表明四只犬的胃中均存在幽门螺杆菌菌株。将PCR产物克隆到大肠杆菌DH10B中，对PCR重新扩增的16S rDNA片段使用限制性内切酶HhaI进行核糖体DNA限制性分析（ARDRA）。限制性图谱表明两只犬中至少存在两种不同的幽门螺杆菌物种。将六个克隆的16S rDNA部分序列与EMBL数据库中的可用序列进行比较。从不同犬获得的两个序列与幽门螺杆菌菌株的相应序列相同。三个序列与幽门螺杆菌显示出最高但中等的相似性值（96.6 - 98.0%），一个序列与所罗门幽门螺杆菌相似（97.3%）。与之前的报道不同，我们的数据表明犬的胃黏膜可能被幽门螺杆菌菌株或与其非常密切相关的物种定植，但也证实了存在迄今未培养的幽门螺杆菌物种的迹象。
