Houseal T W, Dackowski W R, Landes G M, Klinger K W
Integrated Genetics, Framingham, Massachusetts 01701-9322.
Cytometry. 1994 Mar 1;15(3):193-8. doi: 10.1002/cyto.990150303.
The constituents of two cosmid contigs were analyzed by high resolution mapping using two-color fluorescence in situ hybridization (FISH) to extended DNA molecules. Samples were prepared by lysing the nuclei in situ followed by histone depletion. This treatment results in elongate DNA filaments appropriate for high resolution mapping. The hybridization signals appeared as a strong of fluorescent spots separated by non-fluorescing gaps. Probe-specific features of the hybridization patterns were detected and some of the non-fluorescing gaps were found to represent regions of repetitive DNA suppressed during hybridization.
通过使用双色荧光原位杂交（FISH）对延伸的DNA分子进行高分辨率作图，分析了两个黏粒重叠群的组成成分。样品制备方法是先原位裂解细胞核，然后去除组蛋白。这种处理产生适合高分辨率作图的伸长DNA细丝。杂交信号表现为被无荧光间隙隔开的一串强荧光点。检测到杂交模式的探针特异性特征，并且发现一些无荧光间隙代表杂交过程中被抑制的重复DNA区域。
