Parra I, Windle B
Cancer Therapy and Research Center, San Antonio, Texas 78229.
Nat Genet. 1993 Sep;5(1):17-21. doi: 10.1038/ng0993-17.
We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a > 200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.
我们描述了一种拉伸DNA的方法，该方法与荧光杂交程序相结合，形成了一种新的图谱绘制技术，可产生高分辨率、生动的多色图像和图谱。通过该程序成功绘制了限制性片段和黏粒探针，并通过标准限制性图谱分析进行了验证。在两天内轻松生成了一个大于200千碱基区域的长程图谱，该区域包含五个扩增的二氢叶酸还原酶基因拷贝。这种DNA图谱绘制程序为各种标准图谱绘制程序提供了一种重要且快速的替代方法。
