Focà A, Matera G, Berlinghieri M C
Institute of Microbiology, University of Reggio Calabria, Policlinico Mater Domini, Catanzaro, Italy.
Eur J Clin Microbiol Infect Dis. 1993 Dec;12(12):940-4. doi: 10.1007/BF01992169.
Antibiotic-induced endotoxin (lipopolysaccharide; LPS) release may precipitate septic shock. In the present study the effect of teicoplanin, which has been reported to neutralize LPS in experimental models, on LPS neutralization was investigated in human whole blood samples. Levels of interleukin 8, a preinflammatory cytokine which was stimulated by Salmonella minnesota R595 LPS (12.6 micrograms/ml), were monitored over time. Interleukin 8 concentrations increased over time up to 24 h. When LPS was preincubated with teicoplanin (antibiotic: LPS ratio 20:1, w/w), interleukin 8 concentrations were found significantly (p < 0.05) reduced at 4, 8 and 24 h after LPS challenge. Interleukin 1 beta (at 4, 8 and 24 h) and tumor necrosis factor alpha (at 8 and 24 h) levels were also significantly decreased by teicoplanin. In this experiment model, a teicoplanin:LPS ratio 100-fold less than the ratio achievable in plasma of septic shock patients was able to reduce interleukin 8, which has been correlated with the severity of septic disease.
抗生素诱导的内毒素（脂多糖；LPS）释放可能会引发感染性休克。在本研究中，在人全血样本中研究了已报道在实验模型中可中和LPS的替考拉宁对LPS中和的作用。监测了由明尼苏达沙门氏菌R595 LPS（12.6微克/毫升）刺激的促炎细胞因子白细胞介素8的水平随时间的变化。白细胞介素8浓度随时间增加，直至24小时。当LPS与替考拉宁预孵育（抗生素：LPS比例为20:1，w/w）时，发现LPS攻击后4、8和24小时白细胞介素8浓度显著降低（p<0.05）。替考拉宁还显著降低了白细胞介素1β（在4、8和24小时）和肿瘤坏死因子α（在8和24小时）的水平。在该实验模型中，替考拉宁与LPS的比例比感染性休克患者血浆中可达到的比例低100倍，却能够降低与脓毒症严重程度相关的白细胞介素8。
