Ciuffetti G, Mercuri M, Lombardini R, Maragoni G, Santambrogio L, Mannarino E
Second Department of Internal Medicine, University of Perugia, Italy.
J Clin Pathol. 1989 Oct;42(10):1083-7. doi: 10.1136/jcp.42.10.1083.
Whole blood filterability and leucocyte behaviour (number, activation, and subfraction filterability rates) were monitored at the earliest stage of peripheral ischaemia in 18 patients with stage II peripheral occlusive arterial disease (PAOD) and 20 matched controls. A model of controlled ischaemia, using exercise to stress leg circulation, was set up and blood samples were taken before exercise, at the onset of calf pain, and at recovery from peak exercise. Leucocytes were counted, separated into their subfractions on a Ficoll-Hypaque density gradient and by adhesion to Petri dishes, and filtered in buffer (like the whole blood suspensions) through 5 microns pore diameter Nucleopore filters. Unfractionated white cells, separated under gravity, with pseudopodia or cytoplasmic irregularities were regarded as activated. The whole blood filterability rate was significantly increased at the onset of calf pain and was associated with significant increases in the number of leucocytes and in the filterability rate of the monocyte subfraction, the latter persisting throughout the recovery period. No significant changes were observed in the other variables monitored, showing that impairments in white cell rheology may be associated with ischaemia.
在18例II期外周动脉闭塞性疾病（PAOD）患者和20例匹配的对照者外周缺血的最早阶段，监测全血滤过性和白细胞行为（数量、活化及亚群滤过率）。建立了一种可控缺血模型，通过运动使腿部循环受压，并在运动前、小腿疼痛发作时及运动高峰恢复时采集血样。对白细胞进行计数，通过Ficoll-Hypaque密度梯度和贴壁培养将其分离为亚群，并在缓冲液中（如同全血悬液）通过孔径为5微米的核孔滤器进行过滤。在重力作用下分离出的未分级白细胞，若有伪足或细胞质不规则则视为活化。在小腿疼痛发作时，全血滤过率显著增加，且与白细胞数量及单核细胞亚群滤过率的显著增加相关，后者在整个恢复期持续存在。在监测的其他变量中未观察到显著变化，表明白细胞流变学受损可能与缺血有关。
