猪、牛和绵羊（偶蹄目）中一些基因的染色体定位及共线性分析

[Chromosome localization and analysis of synteny analysis of some genes in swine, cattle, and sheep (Artiodactyla)].

作者信息

Kuznetsov S B, Larkin D M, Kaftanovskaia E M, Ivanova E V, Astakhova N M, Cheriakene O V, Zhdanova N S

机构信息

Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia.

出版信息

Genetika. 1998 Sep;34(9):1200-4.

PMID:9879008

Abstract

Using the hybrid cell lines pig-American mink, cow-American mink, and sheep-American mink, the localization of some genes included in a large conservative block localized on human chromosome (chr) 17 was performed by means of electrophoresis of proteins and Southern blot hybridization. Genes NF1, RARA, PRKCA, and ERBB2 were assigned to chr 12 in swine; TK1 and UMPH2, to chr 19 in cattle; and TK1, UMPH2, and PEPA, to chr 11 in sheep. The conserved synteny of these genes in three representatives of the order Artiodactyla was shown.

摘要

利用猪 - 美国水貂、牛 - 美国水貂和羊 - 美国水貂杂交细胞系，通过蛋白质电泳和Southern印迹杂交对位于人类17号染色体上一个大的保守区域中的一些基因进行定位。NF1、RARA、PRKCA和ERBB2基因在猪中定位于12号染色体；TK1和UMPH2基因在牛中定位于19号染色体；TK1、UMPH2和PEPA基因在羊中定位于11号染色体。结果显示了偶蹄目三个代表物种中这些基因的保守同线性。