Lukácová M, Baumann M, Brade L, Mamat U, Brade H
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Germany.
Infect Immun. 1994 Jun;62(6):2270-6. doi: 10.1128/iai.62.6.2270-2276.1994.
In two strains of Chlamydia psittaci and in Chlamydia trachomatis serotype L1, we have detected a so-far-unknown antigen which (i) is resistant to heat and proteolytic digestion, (ii) can be extracted with phenol-water into the water phase, (iii) gives a ladder-like banding pattern in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (iv) is immunogenic in rabbits and mice, and (v) contains immunoreactivity of lipid A, a common and characteristic component of gram-negative lipopolysaccharides (LPS). Thus, chlamydiae contain, in addition to the known rough-type LPS, another LPS type which is phenotypically smooth (S-LPS). S-LPS was observed preferentially in chlamydiae grown in the yolk sac of embryonated eggs; it was, however, also detected by immunofluorescence in tissue culture-grown chlamydiae with a monoclonal antibody against S-LPS.
在两株鹦鹉热衣原体和沙眼衣原体血清型L1中，我们检测到一种迄今未知的抗原，该抗原：（i）耐热且耐蛋白酶消化；（ii）可用酚-水法提取到水相中；（iii）在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中呈现梯状条带模式；（iv）对兔和小鼠具有免疫原性；（v）含有脂多糖A的免疫反应性，脂多糖A是革兰氏阴性脂多糖（LPS）的常见且特征性成分。因此，衣原体除了含有已知的粗糙型LPS外，还含有另一种表型为光滑型的LPS（S-LPS）。S-LPS在鸡胚卵黄囊中生长的衣原体中优先被观察到；然而，用抗S-LPS的单克隆抗体通过免疫荧光法也在组织培养生长的衣原体中检测到了它。
